(-)-Menthol

製品コードS4714 バッチS471401

化学情報

	Synonyms	L-Menthol, Levomenthol, Menthomenthol, Menthacamphor	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₀H₂₀O
	分子量	156.27	CAS No.	2216-51-5
Solubility (25°C)*	体外	DMSO	31 mg/mL (198.37 mM)
		Ethanol	31 mg/mL (198.37 mM)
		Water	16 mg/mL (102.38 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	(-)-Menthol (L-Menthol, Levomenthol, Menthomenthol, Menthacamphor) is a levo isomer of menthol and used as a cooling agent that strongly activates TRPM8.
in vitro	(-)-Menthol induces cytotoxicity against murine leukemia WEHI-3 cells in vitro in a dosedependent manner. (-)-Menthol inhibits the growth of rat liver epithelial tumor cells and acts as a potent chemopreventive agent during DMBA initiation of rat mammary tumors. (-)-menthol inhibits N-acetyltransferase activity. It also is found to inhibit the DNA topoisomerase I, II alpha and beta and to promote NF-kappaB expression in human gastric cancer SNU-5 cells. Additionally, (-)-menthol induces human promyelocytic leukemia HL-60 cell death through the Ca2+ release from the endoplasmic reticulum^[1].
in vivo	The effects of (-)-menthol on WEHI-3 cells in vivo (BALB/c mice) were also examined, and it was observed that the Mac-3 and CD11b markers were decreased, indicating inhibition of differentiation of the precursor of macrophage and granulocyte. The weights of liver and spleen samples from mice treated with (-)-menthol were found to be decreased compared to untreated animals. (-)-Menthol has been shown to be toxic in animals, but in humans, it is considered to be safe, with a small cardio-accelerating effect. At high doses (-)-menthol may exert a depressant effect on the central nervous system in rodents. (-)-menthol statistically decreases the weights of the liver and spleen in the examined animals and also decreases the percentage of MAC-3 and CD11b cells in the blood.^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Murine leukemia cell line (WEHI-3)
	濃度	0, 25, 50, 75 and 100 μM
	反応時間	24 h
	実験の流れ	Approximately 2x10⁵ cells (WEHI-3) are incubated in 12-well plates containing medium for 24 h without (control) or with various concentrations (0, 25, 50, 75 and 100 μM) of (-)-menthol. The cells are harvested and washed with PBS before PI staining. The percentage of viable WEHI-3 cells is determined by trypan blue exclusion and flow cytometry.
動物実験	動物モデル	Male BALB/c mice
	投薬量	1 mg/kg or 10 mg/kg
	投与方法	Oral administration

参考

[1] Lu HF, et al. In Vivo. 2007, 21(2):285-9.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Growth at Cold Temperature Increases the Number of Motor Neurons to Optimize Locomotor Function [ Curr Biol, 2019, 29(11):1787-1799.e5]	PubMed: 31130453

Growth at Cold Temperature Increases the Number of Motor Neurons to Optimize Locomotor Function [ Curr Biol, 2019, 29(11):1787-1799.e5]

PubMed: 31130453

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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