2-Acetylphenothiazine (ML171)

製品コードS5304 バッチS530401

化学情報

	Synonyms	2-APT	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₄H₁₁NOS
	分子量	241.31	CAS No.	6631-94-3
Solubility (25°C)*	体外	DMSO	48 mg/mL (198.91 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	2-Acetylphenothiazine (ML171, 2-APT) is a potent and selective inhibitor of NADPH-oxidase with IC50s of 0.25 μM, 5 μM, 3μM, 5 μM and 5.5 μM for NOX1, NOX2, NOX3, NOX4 and xanthine oxidase, respectively.
in vitro	ML171 is a potent and selective inhibitor of NOX1, with an IC50 of 129–156 nM in cell-based assays. ML171 is not cytotoxic, and is selective among NOX family members NOX2, NOX3, and NOX4, as well as against xanthine oxidase, another cellular source of ROS. In addition, ML171 is highly effective in inhibiting the cellular production of invadopodia in a human colon cancer cell line. It strongly blocks ROS generation in HT29 cells (IC50HT29 = 0.129 μM)^[2]. ML171 does not inhibit mitochondrial ROS production^[3].

製品説明

2-Acetylphenothiazine (ML171, 2-APT) is a potent and selective inhibitor of NADPH-oxidase with IC50s of 0.25 μM, 5 μM, 3μM, 5 μM and 5.5 μM for NOX1, NOX2, NOX3, NOX4 and xanthine oxidase, respectively.

in vitro

ML171 is a potent and selective inhibitor of NOX1, with an IC50 of 129–156 nM in cell-based assays. ML171 is not cytotoxic, and is selective among NOX family members NOX2, NOX3, and NOX4, as well as against xanthine oxidase, another cellular source of ROS. In addition, ML171 is highly effective in inhibiting the cellular production of invadopodia in a human colon cancer cell line. It strongly blocks ROS generation in HT29 cells (IC50HT29 = 0.129 μM)^[2]. ML171 does not inhibit mitochondrial ROS production^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	DLD1 cells
	濃度	10 μM
	反応時間	1 h
	実験の流れ	DLD1 cells were plated on glass coverslips and after 24hrs cells were transfected with active SrcYF or empty vector (mock). 48hrs after transfection, cells were treated as indicated for 1hr with 10 μM of ML171, or with DMSO or 10 μM DPI. Afterwards, cells were fixed and stained and visualized by confocal miscroscopy.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A predicted structure of NADPH Oxidase 1 identifies key components of ROS generation and strategies for inhibition [ PLoS One, 2023, 18(5):e0285206]	PubMed: 37134122
A Histone Deacetylase Inhibitor Induces Acetyl-CoA Depletion Leading to Lethal Metabolic Stress in RAS-Pathway Activated Cells [ Cancers (Basel), 2022, 14(11)2643]	PubMed: 35681624
G Protein-Coupled Estrogen Receptor Agonist G-1 Inhibits Mantle Cell Lymphoma Growth in Preclinical Models [ Front Oncol, 2021, 11:668617]	PubMed: 34211844
Reactive oxygen species mediate thylakoid membrane remodeling and triacylglycerol synthesis under nitrogen starvation in the algaChlorella sorokiniana [ bioRxiv, 2021, 10.1101/2021.05.15.444036]	PubMed: none
Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission. [ ACS Nano, 2020, 24;14(3):3059-3074]	PubMed: 32057235

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。