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Synonyms | 2-bromopalmitate | Storage (From the date of receipt) |
3 years -20°C powder | ||||||||
化学式 | C16H31BrO2 |
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分子量 | 335.32 | CAS No. | 18263-25-7 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 67 mg/mL (199.8 mM) | ||||||||
Ethanol | 67 mg/mL (199.8 mM) | ||||||||||
Water | Insoluble | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | 2-BP (2-Bromohexadecanoic acid) is a nonmetabolisable analogue of palmitate and acts as a palmitoylation inhibitor, which inhibits multiple palmitoyl-acyl transferase (PAT) activity towards MyrGCK(NBD) and FarnCNRas(NBD) with identical IC50s of approx. 4 µM. 2-Bromohexadecanoic acid can directly and irreversibly inhibit the palmitoyltransferase activity of all DHHC (Asp-His-His-Cys) proteins. |
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in vitro | 2-Bromohexadecanoic acid induces the interactions between GSDME-N and GSDME-C, and inhibits the GSDME-C palmitoylation, exhibiting chemotherapy induced pyroptosis.[1] |
細胞アッセイ | 細胞株 | Hela cells |
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濃度 | 50 μM | |
反応時間 | 3, 6, 9, 12 h | |
実験の流れ | Cells are pre-seeded overnight until cell density reached ~60%. The culture medium is switched to fresh medium containing relevant drugs and incubated for a period of time as indicated. After 2-BP and TNFα+CHX treatment, Hela cells are collected at the indicated time points and stained with FITC-Annexin V and PI, and subjected to Hoechst 33342 and PI double staining. The culture supernatants are collected to measure the percentage of LDH release. |
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Hepatitis E virus ORF3 protein hijacking thioredoxin domain-containing protein 5 (TXNDC5) for its stability to promote viral particle release [ J Virol, 2024, 98(4):e0164923] | PubMed: 38548704 |
ZDHHC2-Mediated AGK Palmitoylation Activates AKT-mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell Carcinoma [ Cancer Res, 2023, 83(12):2034-2051] | PubMed: 37078777 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。