4-PBA (4-Phenylbutyric acid)

製品コードS3592 バッチS359203

印刷

化学情報

Chemical Structure Synonyms Benzenebutyric acid Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C10H12O2
分子量 164.20 CAS No. 1821-12-1
Solubility (25°C)* 体外 DMSO 33 mg/mL (200.97 mM)
Ethanol 33 mg/mL (200.97 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 4-PBA (4-Phenylbutyric acid) is a histone deacetylase (HDAC) inhibitor and a key epigenetic inducer of anti-HCV hepatic hepcidin. 4-Phenylbutyric acid inhibits LPS-induced inflammation through regulating endoplasmic-reticulum (ER) stress and autophagy in acute lung injury models.
in vitro

4-PBA inhibits the ER stress induced by SiNPs in RAW264.7 cells, as evidenced by the expansion and degranulation of ER, as well as greatly up-regulated Bip and CHOP expressions.[3]
in vivo

4-PBA attenuates LPS-induced bone loss in lipopolysaccharide (LPS)-treated mice, by increasing area of TRAP-positive osteoclasts (OCs) and serum level of collagen type I fragments.[4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Mouse macrophage cell line, RAW264.7 cells
濃度 3 mM
反応時間 6 h
実験の流れ

Mouse macrophage cell line, RAW264.7 cells are cultured in DMEM with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. SiNPs are diluted by DMEM to appropriate concentrations, and an ER stress inhibitor, 4-phenylbutyric acid (4-PBA) is applied (3 mM, 6 h). The SiNPs-treated group is at a concentration of 50 μg/ml in vitro experiments. Similarly, the application of 4-PBA is set up through MTT assay, after SiNPs with or without 4-PBA treatment, cells are harvested for the following measurement. The levels of Bip and CHOP induced by SiNPs are measured.
動物実験 動物モデル mice are injected daily intraperitoneally in 200 μl PBS (or with PBS as a vehicle) at a dose of 240 mg/kg for 3 weeks.
投薬量 i.p.
投与方法

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A novel CSN5/CRT O-GlcNAc/ER stress regulatory axis in platinum resistance of epithelial ovarian cancer [ Int J Biol Sci, 2024, 20(4):1279-1296] PubMed: 38385070
Inhibition of OGFOD1 by FG4592 confers neuroprotection by activating unfolded protein response and autophagy after ischemic stroke [ J Transl Med, 2024, 22(1):248] PubMed: 38454480
IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties [ Cell Mol Biol Lett, 2023, 28(1):15] PubMed: 36849929
Porcine reproductive and respiratory syndrome virus infection triggers autophagy via ER stress-induced calcium signaling to facilitate virus replication [ PLoS Pathog, 2023, 19(3):e1011295] PubMed: 36972295
Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells [ Int J Mol Sci, 2023, 24(11)9640] PubMed: 37298591
Large HBV Surface Protein-Induced Unfolded Protein Response Dynamically Regulates p27 Degradation in Hepatocellular Carcinoma Progression [ Int J Mol Sci, 2023, 10.3390/ijms241813825] PubMed: 37762128
Endoplasmic reticulum stress mediates environmental particle-induced inflammatory response in bronchial epithelium [ J Immunotoxicol, 2023, 10.1080/1547691X.2023.2229428] PubMed: 37417782
Effects of endoplasmic reticulum stress on erectile function in rats with cavernous nerve injury [ Sex Med, 2023, 11(4):qfad050] PubMed: 37674767
ASK1-ER stress pathway-mediated fibrotic-EV release contributes to the interaction of alveolar epithelial cells and lung fibroblasts to promote mechanical ventilation-induced pulmonary fibrosis [ Exp Mol Med, 2022, 1-13.] PubMed: 36473935
A heterozygous p.S143P mutation in LMNA associates with proteasome dysfunction and enhanced autophagy-mediated degradation of mutant lamins A and C [ Front Cell Dev Biol, 2022, 10:932983] PubMed: 36111332

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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