7,12-Dimethylbenz[a]anthracene (DMBA)

製品コードE1022 バッチE102204

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C20H16
分子量 256.34 CAS No. 57-97-6
Solubility (25°C)* 体外 DMSO 14 mg/mL (54.61 mM)
Ethanol 4 mg/mL (15.6 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 7,12-Dimethylbenz[a]anthracene (DMBA) is an immunosuppressor as well as a potent organ-specific carcinogen.
in vitro

In splenocytes, which is isolated from mice, lymphocyte function is completely suppressed by the treatment with 50 μM DMBA. The susceptibility of splenocytes isolated from mEH-null mice is lower than from wildtype mice on the immunosuppression activity of DMBA.[1]
in vivo

The metabolic activation of DMBA in the spleen plays a key role in the splenic immunotoxicity, mEH-null mice are more resistant to DMBA-induced cytotoxicity as well as immunosuppression.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Splenocytes
濃度 10 μM, 20 μM, 50 μM
反応時間 --
実験の流れ

Spleens are harvested and placed in a sterile phosphatebuffered saline. Cell suspensions are prepared by gentle dissociation using glass slides. Debris is separated from cells by filtration and cell suspensions are centrifuged at 250 x g for 5 min. Cells are seeded in a volume of 100 µl in 96-well plates. Mitogens were added in a volume of 10 µl at final concentrations of 25 µg/ml for LPS and 5 µg/ml for PHA, and then the cells were incubated in 5% CO2 at 37°C for 3 days. The cells were harvested onto filter strips using a harvester and [3 H]-thymidine incorporation was determined by liquid scintillation. For mitogen-induced lymphocyte proliferation assays after DMBA treatment in vitro, cell preparations from untreated mice were simultaneously exposed to mitogen (LPS) and DMBA.
動物実験 動物モデル mEH-null mice
投薬量 30 mg/kg, 100 mg/kg
投与方法 i.p.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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