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Synonyms | 740YPDGFR | Storage (From the date of receipt) |
3 years -20°C powder (seal) | ||||
化学式 | C141H222N43O39PS3 |
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分子量 | 3270.70 | CAS No. | 1236188-16-1 | ||||
Solubility (25°C)* | 体外 | DMSO | 100 mg/mL (30.57 mM) | ||||
Water | 8 mg/mL (2.44 mM) | ||||||
Ethanol | Insoluble | ||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | 740 Y-P (PDGFR 740Y-P, 740YPDGFR) is a cell-permeable phosphopeptide activator of PI3K. |
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in vitro | The PDGFR 740Y-P peptide stimulates a mitogenic response in muscle cells. The ability of the 740Y-P peptide to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[2]. 740Y-P is as effective as a growth factor (FGF2) at promoting neuronal cell survival via the established PI 3-kinase-Akt survival cascade. Stimulation of neuronal cell survival by the PDGFR740Y-P peptide is dose dependent and does not require insulin[1]. |
in vivo | 740 Y-P decreases the degree of ROS levels in Aβ(25-32) treated hippocampal tissues and increases the extent of AKT and PI3K phosphorylation in Alzheimer’s disease rat model. |
細胞アッセイ | 細胞株 | NIH 3T3 cells |
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濃度 | 50 μg/ml | |
反応時間 | 2 h | |
実験の流れ | NIH 3T3 cells (2×106) are incubated in suspension at 37°C in Ca2+ and Mn2+ free HBSS, 10% FCS containing either 50 μg/ml of 740Y-P peptide or an equal volume of PBS as a control. After 2h cells are centrifuged, washed and trypsinised to degrade non-internalised peptide. Cells are then resuspended in lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 10% Glycerol, 2% NP40, 0.25% deoxycholate, 1 mM EDTA, 1 mM Vanadate, Protease inhibitors "complete" cocktail from Boehringer-Mannheim) for 1 h at 4°C. Lysates are clarified by centrifugation at 1.4×104g for 5 min and the supernatants are incubated for 1h with streptavidin-agarose beads. Beads are subsequently washed three times in lysis buffer and finally boiled in SDS sample buffer and resolved by SDS-PAGE on a 12% gel, transferred to nitrocellulose and immunoblotted with the p85 monoclonal antibody. |
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動物実験 | 動物モデル | Male Sprague dawely rats |
投薬量 | 10 mg/kg | |
投与方法 | i.p. |
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Efficient generation of induced pluripotent stem cell lines from healthy donors' peripheral blood mononuclear cells of different genders [ Stem Cell Res, 2024, 77:103421] | PubMed: 38636268 |
Effects of Cyanobacterial Harmful Algal Bloom Toxin Microcystin-LR on Gonadotropin-Dependent Ovarian Follicle Maturation and Ovulation in Mice [ Environ Health Perspect, 2023, 131(6):67010] | PubMed: 37342990 |
Effects of Cyanobacterial Harmful Algal Bloom Toxin Microcystin-LR on Gonadotropin-Dependent Ovarian Follicle Maturation and Ovulation in Mice [ Environ Health Perspect, 2023, 131(6):67010] | PubMed: 37342990 |
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Notum enhances gastric cancer stem-like cell properties through upregulation of Sox2 by PI3K/AKT signaling pathway [ Cell Oncol (Dordr), 2023, 10.1007/s13402-023-00875-w] | PubMed: 37749430 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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