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Synonyms | NSC 646666, Natural Yellow 18 chloride | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C20H18NO4.Cl |
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分子量 | 371.81 | CAS No. | 633-65-8 | |
Solubility (25°C)* | 体外 | DMSO | 40 mg/mL (107.58 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Berberine chloride is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator. |
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in vitro | Compared with regorafenib alone, the combined treatment of Berberine (BBR) and regorafenib significantly inhibits the proliferation of hepatocellular carcinoma (HCC) cells and induces cellular apoptosis.[3] |
in vivo | The combined treatment group with Berberine (BBR) and regorafenib has a dramatic inhibitory effect on the growth of hepatocellular carcinoma (HCC) xenograft tumors in nude mice. The increased apoptosis of xenograft tumors is seen in the combined treatment group.[3] |
細胞アッセイ | 細胞株 | hepatocellular carcinoma (HCC) cells |
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濃度 | 5, 10, 20, 40, 80, 160, 320, 640 and 1280 μM | |
反応時間 | 48 h | |
実験の流れ | HCC cells are seeded into a 96-well plate with 5,000 cells per well. After overnight incubation, HCC cells are treated with the indicated concentrations of regorafenib, Berberine (BBR) or their combination for 24h or 48h. The viability of HCC is examined by MTS Assay and Synergy H1/Epoch microplate reader.The interaction between regorafenib and BBR is quantified by Com-puSyn software. The Cell-LightTM EdU ApolloR567 In Vitro Imaging Kit is used to detect the level of cell proliferation. The Annexin V-FITC/PI Apoptosis Detection Kit and DeadEndTM Fluorometric Tunel System assay are used to measure cellular apoptotic level. |
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動物実験 | 動物モデル | Male BALB/c nude mice |
投薬量 | 10mg/kg/day | |
投与方法 | i.p. (Data sourced from selleck products) |
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Repurposing FDA-Approved Drugs for Temozolomide-Resistant IDH1 Mutant Glioma Using High-Throughput Miniaturized Screening on Droplet Microarray Chip [ Adv Healthc Mater, 2023, e2300591.] | PubMed: 37162029 |
Connexin43 is associated with the progression of clear cell renal carcinoma and is regulated by tangeretin to sygergize with tyrosine kinase inhibitors [ Transl Oncol, 2023, 35:101712] | PubMed: 37354638 |
Berberine chloride (dual topoisomerase I and II inhibitor) modulate mitochondrial uncoupling protein (UCP1) in molecular docking and dynamic with in-vitro cytotoxic and mitochondrial ATP production [ J Biomol Struct Dyn, 2022, 1-11] | PubMed: 35612892 |
Berberine Sensitizes Human Hepatoma Cells to Regorafenib via Modulating Expression of Circular RNAs [ Front Pharmacol, 2021, 12:632201] | PubMed: 34220494 |
Divergent and Overlapping Roles for Selected Phytochemicals in the Regulation of Pathological Cardiac Hypertrophy [ Molecules, 2021, 26(5)1210] | PubMed: 33668293 |
Quantitative Proteomics Analysis of Berberine-Treated Colon Cancer Cells Reveals Potential Therapy Targets [ Biology (Basel), 2021, 10(3)250] | PubMed: 33806918 |
Suppression effect of plant-derived berberine on cyprinid herpesvirus 2 proliferation and its pharmacokinetics in Crucian carp (Carassius auratus gibelio) [ Antiviral Res, 2020, 186:105000] | PubMed: 33359191 |
Berberine, a natural plant alkaloid, synergistically sensitizes human liver cancer cells to sorafenib. [ Oncol Rep, 2018, 40(3):1525-1532] | PubMed: 30015938 |
Characterization of glycosaminoglycan interaction sites and exosite inhibitors of cathepsin K [Haoran Cui, et al. Shandong University, 2014, Haoran Cui] |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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