Capecitabine

製品コードS1156 バッチS115604

化学情報

	Synonyms	RO 09-1978	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₅H₂₂FN₃O₆
	分子量	359.35	CAS No.	154361-50-9
Solubility (25°C)*	体外	DMSO	72 mg/mL (200.36 mM)
		Ethanol	72 mg/mL (200.36 mM)
		Water	6 mg/mL (16.69 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Capecitabine is a tumor-selective fluoropyrimidine carbamate, which achieves higher intratumoral 5-FU level with lower toxicity than 5-FU. Capecitabine treatment of HCT-15 cells causes condensation of DNA and induces apoptosis.
in vitro	Both LS174T WT and LS174T-c2 cells show significantly greater sensitivity to Capecitabine when cultivated in the same plates as HepG2 hepatoma with IC50 values of 890 and 630 μM in LS174T WT alone and cultivated with HepG2, respectively. In addition, for the LS174T-C2 subline, the IC50 falls from 330 ± 4 down to 89 ± 6 μm when cultivated in the same plates as hepatoma cells. Furthermore, Capecitabine induces apoptosis in a Fas-dependent manner, and shows a 7-fold higher cytotoxicity and markedly stronger apoptotic potential in thymidine phosphorylase (TP)-transfected LS174T-c2 cells. ^[1]
in vivo	In the human cancer xenograft models studied, Capecitabine is more effective in a wider dose range and has a broader spectrum of antitumor activity than 5-FU, UFT or its intermediate metabolite 5'-DFUR, which can be correlated with tumor dThdPase levels. ^[2] Capecitabine inhibits tumor growth and metastatic recurrence after resection of human hepatocellular carcinoma (HCC) in highly metastatic nude mice model which is attributed to the high expression of platelet-derived endothelial cell growth factor in tumors. ^[3]
特徴	A tumor-selective fluoropyrimidine carbamate.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HepG2, LS174T WT and LS174T-c2 cells
	濃度	~1 mM
	反応時間	72 hours
	実験の流れ	HepG2 and either LS174T WT or LS174T-c2 cells are seeded, respectively, in the top and bottom chambers of 8-well strip membranes in 96-well plates. The exponentially growing cells are exposed to increasing concentrations of capecitabine. The medium is supplemented with 750 ng/mL ZB4 MoAB or 100 ng/mL BR17 MoAB when the latter are used in the experiments. After 72 hours of continuous exposure, LS174T viability is assessed using the classic colorimetric MTT test.
動物実験	動物モデル	BALB/c nu/nu mice are inoculated s.c. with small pieces of CXF280 xenograft tissues
	投薬量	≤1.5 mM/kg/day
	投与方法	Administered via p.o.

参考

カスタマーフィードバック

: , , Cancer Res, 2017, 77(24):7120-7130

: Data independently produced by , , Dr. Helen Sadik of Johns Hopkins University

: Data from [Data independently produced by , , Carcinogenesis, 2018, 39(1):72-83]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway [ Genes Dis, 2024, 11(1):430-448]	PubMed: 37588209
Establishment and Characterization of NCC-PMP1-C1: A Novel Patient-Derived Cell Line of Metastatic Pseudomyxoma Peritonei [ J Pers Med, 2022, 12(2)258]	PubMed: 35207746
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Establishment and characterization of novel patient-derived cell lines from giant cell tumor of bone [ Hum Cell, 2021, 10.1007/s13577-021-00579-z]	PubMed: 34304386
Establishment and characterization of the NCC-GCTB4-C1 cell line: a novel patient-derived cell line from giant cell tumor of bone [ Hum Cell, 2021, 10.1007/s13577-021-00639-4]	PubMed: 34731453
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Hydrogel-based colorectal cancer organoid co-culture models [ Acta Biomater, 2020, S1742-7061(20)30751-0]	PubMed: 33388439
IRE1α-targeting downregulates ABC transporters and overcomes drug resistance of colon cancer cells. [ Cancer Lett, 2020, 28;476:67-74]	PubMed: 32061752
Establishment and characterization of patient-derived cancer models of malignant peripheral nerve sheath tumors. [ Cancer Cell Int, 2020, 19;20:58]	PubMed: 32099531

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