Hematoxylin

製品コードS2384 バッチS238402

化学情報

	Synonyms	Hydroxybrazilin	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₆H₁₄O₆
	分子量	302.28	CAS No.	517-28-2
Solubility (25°C)*	体外	DMSO	60 mg/mL (198.49 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.
in vitro	1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change. 2. Hydrate the samples as follows. i. Transfer the slides through three changes of 100% ethanol for 2 min per change. ii. Transfer to 95% ethanol for 2 min. iii. Transfer to 70% ethanol for 2 min. iv. Rinse the slides in running tap water at room temperature for at least 2 min. 3. Stain the samples in Hematoxylin solution for 3 min. 4. Place the slides under running tap water at room temperature for at least 5 min. 5. Stain the samples in working eosin Y solution for 2 min. 6. Dehydrate the samples as follows. i. Dip the slides in 95% ethanol about 20 times. ii. Transfer to 95% ethanol for 2 min. iii. Transfer through two changes of 100% ethanol for 2 min per change. 7. Clear the samples in three changes of xylene for 2 min per change. 8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.

製品説明

Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.

in vitro

1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change.
2. Hydrate the samples as follows.
i. Transfer the slides through three changes of 100% ethanol for 2 min per change.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer to 70% ethanol for 2 min.
iv. Rinse the slides in running tap water at room temperature for at least 2 min.
3. Stain the samples in Hematoxylin solution for 3 min.
4. Place the slides under running tap water at room temperature for at least 5 min.
5. Stain the samples in working eosin Y solution for 2 min.
6. Dehydrate the samples as follows.
i. Dip the slides in 95% ethanol about 20 times.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer through two changes of 100% ethanol for 2 min per change.
7. Clear the samples in three changes of xylene for 2 min per change.
8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.

プロトコル(参考用のみ)

参考

[1] Godwin A, J Pharm Clin Sci, 2011, 1, 24-34.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。