Hydroxyurea

製品コードS1896 バッチS189605

化学情報

	Synonyms	NCI-C04831, Hydroxycarbamide,NSC-32065	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	CH₄N₂O₂
	分子量	76.05	CAS No.	127-07-1
Solubility (25°C)*	体外	DMSO	15 mg/mL (197.23 mM)
		Water	15 mg/mL (197.23 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection.
in vitro	hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants^[1]^[2].hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase^[1].

製品説明

Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection.

in vitro

hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants^[1]^[2].hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Erythroid cells
	濃度	30 μM
	反応時間	96 h
	実験の流れ	Erythroid cells obtained from peripheral blood of the same patients(Thirteen β-Thal/HbE patients are treated with hydroxyurea orally for 2 years at a starting dose of 5 mg/kg/day for 5 days/week with escalation to a maximum of 10 mg/kg/day) 1 year after they had stopped hydroxyurea treatment are treated with hydroxyurea in vitro.Treatment of cells performs in primary culture with 30 μM hydroxyurea for 96 hours.
動物実験	動物モデル	Female athymic (nu/nu) nude mice(used for xenograft model)
	投薬量	1500 mg/kg
	投与方法	i.p.

参考

[4] Boucher PD, et al. Gene Ther. 2002, 9(15):1023-30.

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カスタマーフィードバック

: Data from [Data independently produced by , , Blood, 2017, 130(26):2848-2859]

: Data from [Data independently produced by , , Int J Biochem Cell Biol, 2018, 105:1-12]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

CDC5L surveils cellular stress responses and stress granule formation through transcriptional repression [ bioRxiv, 2024, 10.1101/2024.01.04.574133]	PubMed: none
Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish [ Cell Rep, 2023, 42(7):112660]	PubMed: 37342912
Targeting MCL-1 triggers DNA damage and an anti-proliferative response independent from apoptosis induction [ Cell Rep, 2023, 42(10):113176]	PubMed: 37773750
Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis [ Sci Adv, 2023, 9(4):eadf6277]	PubMed: 36706191
Genetic instability from a single S phase after whole-genome duplication [ Nature, 2022, 604(7904):146-151]	PubMed: 35355016
Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity [ Cell Res, 2022, 10.1038/s41422-022-00731-w]	PubMed: 36280710
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
Inactivation of the Hippo tumor suppressor pathway promotes melanoma [ Nat Commun, 2022, 13(1):3732]	PubMed: 35768444
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
Synthetic lethality between TP53 and ENDOD1 [ Nat Commun, 2022, 13(1):2861]	PubMed: 35606358

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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