Neomycin sulfate

製品コードS2568 バッチS256803

化学情報

	Synonyms	Framycin sulfate	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₃H₄₆N₆O₁₃.H₂SO₄
	分子量	712.72	CAS No.	28002-70-2 ,1405-10-3
Solubility (25°C)*	体外	Water	100 mg/mL (140.3 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Neomycin sulfate(Framycin sulfate) is an aminoglycoside antibiotic, used to treat bacteria infections.
in vitro	Neomycin interacts preferentially with the ribozyme-substrate complex and that this interaction leads to a reduction in the cleavage rate by stabilizing the ground state of the complex and destabilizing the transition state of the cleavage step. ^[1] Neomycin effects a conformational change in the structure of trans-activating region (TAR) that can be detected by circular dichroism spectroscopy. Neomycin acts as a noncompetitive inhibitor that can bind to the Tat-TAR complex and increase the rate constant (koff) for dissociation of the peptide from the RNA. ^[2] Neomycin is the most effective aminoglycoside (groove binder) in stabilizing a DNA triple helix. Neomycin stabilizes TAT, as well as mixed base DNA triplexes, better than known DNA minor groove binders (which usually destabilize the triplex) and polyamines. Neomycin shows a preference for stabilization of TAT triplets but can also accommodate CGC(+) triplets. ^[3] Neomycin induces a concentration- and voltage-dependent partial block from both the cytosolic and luminal faces of the channel. Neomycin has a greater affinity for the luminal site of interaction than the cytosolic site: zero-voltage dissociation constants (Kb(0)) are respectively 210.20 nM and 589.70 nM for luminal and cytosolic block. Neomycin also exhibits voltage-dependent relief of block at holding potentials >+60 mV. ^[4]

製品説明

Neomycin sulfate(Framycin sulfate) is an aminoglycoside antibiotic, used to treat bacteria infections.

in vitro

Neomycin interacts preferentially with the ribozyme-substrate complex and that this interaction leads to a reduction in the cleavage rate by stabilizing the ground state of the complex and destabilizing the transition state of the cleavage step. ^[1] Neomycin effects a conformational change in the structure of trans-activating region (TAR) that can be detected by circular dichroism spectroscopy. Neomycin acts as a noncompetitive inhibitor that can bind to the Tat-TAR complex and increase the rate constant (koff) for dissociation of the peptide from the RNA. ^[2] Neomycin is the most effective aminoglycoside (groove binder) in stabilizing a DNA triple helix. Neomycin stabilizes TAT, as well as mixed base DNA triplexes, better than known DNA minor groove binders (which usually destabilize the triplex) and polyamines. Neomycin shows a preference for stabilization of TAT triplets but can also accommodate CGC(+) triplets. ^[3] Neomycin induces a concentration- and voltage-dependent partial block from both the cytosolic and luminal faces of the channel. Neomycin has a greater affinity for the luminal site of interaction than the cytosolic site: zero-voltage dissociation constants (Kb(0)) are respectively 210.20 nM and 589.70 nM for luminal and cytosolic block. Neomycin also exhibits voltage-dependent relief of block at holding potentials >+60 mV. ^[4]

プロトコル(参考用のみ)

参考

[4] Mead F, et al. Biophys J,?002, 82(4), 1953-1963.

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes. [ Mol Cell, 2020, 22 pii: S1097-2765(20)30231-8]	PubMed: 32353258

Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes. [ Mol Cell, 2020, 22 pii: S1097-2765(20)30231-8]

PubMed: 32353258

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人間や獣医の診断であるか治療的な使用のためにでない。

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