受注:045-509-1970 |
技術サポート:[email protected] 平日9:00〜18:00 1営業日以内にご連絡を差し上げます |
Synonyms | CL13900 2HCl | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
|
化学式 | C22H29N7O5.2HCl |
|||
分子量 | 544.43 | CAS No. | 58-58-2 | |
Solubility (25°C)* | 体外 | Water | 100 mg/mL warmed with 50ºC water bath (183.67 mM) | |
DMSO | 14 mg/mL warmed with 50ºC water bath (25.71 mM) | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation. |
---|---|
in vitro | The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1] Puromycin can be used for the selection of recombinant cells from noncultured cells. [2] |
in vivo | Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.[4] |
細胞アッセイ | 細胞株 | HepG2 cells |
---|---|---|
濃度 | 1.6 μM | |
反応時間 | 72 h | |
実験の流れ | Cells were treated with different concentrations of drug for 72 h. |
|
動物実験 | 動物モデル | Male Sprague-Dawley rats |
投薬量 | 5 mg/100 g | |
投与方法 | i.v. |
The dopamine receptor D1 inhibitor, SKF83566, suppresses GBM stemness and invasion through the DRD1-c-Myc-UHRF1 interactions [ J Exp Clin Cancer Res, 2024, 43(1):25] | PubMed: 38246990 |
Hyperactivation of β-catenin signal in hepatocellular carcinoma recruits myeloid-derived suppressor cells through PF4-CXCR3 axis [ Cancer Lett, 2024, 586:216690] | PubMed: 38307410 |
DHCR7 promotes lymph node metastasis in cervical cancer through cholesterol reprogramming-mediated activation of the KANK4/PI3K/AKT axis and VEGF-C secretion [ Cancer Lett, 2024, 584:216609] | PubMed: 38211648 |
SHMT2 promotes papillary thyroid cancer metastasis through epigenetic activation of AKT signaling [ Cell Death Dis, 2024, 15(1):87] | PubMed: 38272883 |
MDM2 provides TOP2 poison resistance by promoting proteolysis of TOP2βcc in a p53-independent manner [ Cell Death Dis, 2024, 15(1):83] | PubMed: 38263255 |
METTL3 facilitates renal cell carcinoma progression by PLOD2 m6A-methylation under prolonged hypoxia [ Cell Death Dis, 2024, 15(1):62] | PubMed: 38233403 |
Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers [ Cell Death Dis, 2024, 15(2):149] | PubMed: 38365764 |
Dual roles of CCDC102A in governing centrosome duplication and cohesion [ Cell Rep, 2024, 43(2):113696] | PubMed: 38280197 |
PLEKHM2 deficiency induces impaired mitochondrial clearance and elevated ROS levels in human iPSC-derived cardiomyocytes [ Cell Death Discov, 2024, 10(1):142] | PubMed: 38490981 |
Transcriptomic analysis of the hypoxia-inducible factor 1α impact on the gene expression profile of chicken fibroblasts under hypoxia [ Poult Sci, 2024, 103(3):103410] | PubMed: 38277890 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。