TAME

製品コードS2225 バッチS222501

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₄H₂₂N₄O₄S
	分子量	342.41	CAS No.	901-47-3
Solubility (25°C)*	体外	DMSO	69 mg/mL (201.51 mM)
		Water	69 mg/mL (201.51 mM)
		Ethanol	3 mg/mL (8.76 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Tosyl-L-Arginine Methyl Ester (TAME) is an APC inhibitor.
in vitro	TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 µM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. ^[1] TAME is hydrolysed by trypsin with K_m of 0.328 mM. ^[2] TAME accelerates the ATP hydrolysis process about 12-fold. ^[3] TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. ^[4] TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. ^[5] TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 10³ M. ^[6]

製品説明

Tosyl-L-Arginine Methyl Ester (TAME) is an APC inhibitor.

in vitro

TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 µM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. ^[1] TAME is hydrolysed by trypsin with K_m of 0.328 mM. ^[2] TAME accelerates the ATP hydrolysis process about 12-fold. ^[3] TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. ^[4] TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. ^[5] TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 10³ M. ^[6]

プロトコル(参考用のみ)

キナーゼアッセイ	³H-TAME binding assay
キナーゼアッセイ	³H-TAME (200 nM; 15 Ci/mmol) is added to 100 μl interphase Xenopus extract or HeLa cell lysate. APC is immunoprecipitated with Cdc27 antibody coupled to affiprep beads. The beads are washed with XB and radioactivity measured by scintillation counting. Alternatively, ³H-TAME is added and Cdc27 immunoprecipitation is performed after one or two rounds of APC immunodepletion. Specific binding is calculated as the difference between counts associated with Cdc27 antibody beads compared to beads lacking antibody (mock IP

参考

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カスタマーフィードバック

: Data from [Data independently produced by , , Oncotarget, 2016, 7(18):25478-92]

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