ACSS2 inhibitor

製品コードS8588 バッチS858803

印刷

化学情報

Chemical Structure Synonyms Ac-CoA Synthase Inhibitor1 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C20H18N4O2S2
分子量 410.51 CAS No. 508186-14-9
Solubility (25°C)* 体外 DMSO (warmed with 50ºC water bath) 82 mg/mL (199.75 mM)
Ethanol 5 mg/mL (12.17 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO Corn oil
1.03mg/ml Taking the 1 mL working solution as an example, add 50 μL of 20.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 40% PEG 300 55%ddH2O
1.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 550 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 ACSS2 inhibitor is the most potent and specific inhibitor of acetate-dependent acetyl-CoA synthetase 2 (ACSS2). ACSS2 inhibitor also inhibits the respiratory syncytial virus (RSV).
in vitro

This quinoxaline compound inhibits the ability of HepG2 cells to incorporate [14C]acetate into lipids with IC50 = 6.8 μM. The quinoxaline is also able to inhibit HepG2 utilization of [14C]acetate for histone acetylation with IC50 = 5.5 μM[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 MDA-MB-231-R cells
濃度 10 uM
反応時間 6 h
実験の流れ

10 µM of ACSS2 inhibitor was added in flag-WT or KR-STAT3 expressing MDA-MB-231-R cells and incubated for 6 h.
動物実験 動物モデル Female C57BL/6J mice
投薬量 15 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence [ Nat Commun, 2024, 15(1):3200] PubMed: 38615014
Acetyl-CoA synthetase 2 induces pyroptosis and inflammation of renal epithelial tubular cells in sepsis-induced acute kidney injury by upregulating the KLF5/NF-κB pathway [ Cell Commun Signal, 2024, 22(1):187] PubMed: 38515158
Sodium benzoate induces pancreatic inflammation and β cell apoptosis partially via benzoylation [ Ecotoxicol Environ Saf, 2024, 270:115877] PubMed: 38150747
Pyruvate metabolism controls chromatin remodeling during CD4+ T cell activation [ Cell Rep, 2023, 42(6):112583] PubMed: 37267106
Pyruvate metabolism controls chromatin remodeling during CD4+ T cell activation [ Cell Rep, 2023, 42(6):112583] PubMed: 37267106
Activation of acetyl-CoA synthetase 2 mediates kidney injury in diabetic nephropathy [ JCI Insight, 2023, 10.1172/jci.insight.165817] PubMed: 37870960
Fatty acid oxidation protects cancer cells from apoptosis by increasing mitochondrial membrane lipids [ Cell Rep, 2022, 39(9):110870] PubMed: 35649368
Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells [ Elife, 2022, 11e75908] PubMed: 36052997
ATP citrate lyase links increases in glycolysis to diminished release of vesicular suppressor of cytokine signaling 3 by alveolar macrophages [ Biochim Biophys Acta Mol Basis Dis, 2022, 1868(10):166458] PubMed: 35700791
Metabolic Adaptation as Potential Target in Papillary Renal Cell Carcinomas Based on Their In Situ Metabolic Characteristics [ Int J Mol Sci, 2022, 23(18)10587] PubMed: 36142502

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。