α-Hederin

製品コードS3914 バッチS391401

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C41H66O12
分子量 750.96 CAS No. 27013-91-8
Solubility (25°C)* 体外 DMSO 100 mg/mL (133.16 mM)
Water Insoluble
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 α-hederin is a water-soluble pentacyclic triterpenoid saponin which has shown hemolytic and apoptotic properties.
in vitro α-hederin shows strong inhibitory activity on the growth of breast cancer cells and induces apoptosis in these cells. α-hederin induces depolarization of mitochondrial membrane potential which releases Apaf-1 and cytochrome c from the intermembrane space into the cytosol, where they promote caspase-3 and caspase-9 activation[1].
in vivo After a single i.v. (2 mg/kg) administration, the mean plasma clearance (CL), volume of distribution (VSS) and elimination half-life (t1/2) of α-hederin in rtas are 0.24 L/h/kg, 0.25 L/kg and 2.67 h, respectively. The oral bioavailability (F) of α-hederin in rats is about 0.14%, which might result from the poor intestinal absorption and/or extensive biliary excretion. α-Hederin decreases the hepatotoxicity of cadmium in mice by inducing hepatic metallothionein I/II, and the mechanism partly involved the upregulation of metal-lothionein expression mediated by TNF-α and IL-6[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 The human breast cancer cell lines MCF-7 and MDA-MB-231
濃度 0.08, 0.4, 2 and 10 μg/ml
反応時間 12, 24 and 48 h
実験の流れ The MTT assay is used to measure the inhibition of growth by α-hederin in breast cancer cell lines. Briefly, 5×103 cells are seeded into a 96-well plate in triplicate and 8 h later α-hederin is added into the wells at the indicated final concentrations (0.08, 0.4, 2 and 10 μg/ml), while cells cultured in medium with 0.05% DMSO as a negative control. After incubation with α-hederin for 12, 24 and 48 h, the medium in each well is replaced with 20 μl of MTT at 5 mg/ml final concentration, and 4 h later 150 μl DMSO/well is added to dissolve the formed violet formazan crystals within metabolically viable cells. The plates are incubated at room temperature for 15 min and then read at 490 nm with a microplate reader. The percentage of growth inhibition is calculated as (OD of the control-OD of the experiment samples)/OD of the control × 100.
動物実験 動物モデル Sprague-Dawley (SD) rats
投薬量 10 mg/kg (p.o.) or 2 mg/kg (i.v.)
投与方法 p.o. or i.v.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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