AOA (Aminooxyacetic acid) hemihydrochloride

製品コードS4989 バッチS498903

印刷

化学情報

Chemical Structure Synonyms Carboxymethoxylamine hemihydrochloride, AOAA hemihydrochloride, Aminooxyacetate hemihydrochloride Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C2H5NO3.1/2HCl
分子量 109.3 CAS No. 2921-14-4
Solubility (25°C)* 体外 DMSO 22 mg/mL (201.28 mM)
Water 22 mg/mL (201.28 mM)
Ethanol 6 mg/mL (54.89 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 AOA (Aminooxyacetic acid) hemihydrochloride is an inhibitor of aminobutyrate aminotransferase involved in amino acid and polyamine metabolism. AOA hemihydrochloride is also a CBS (cystathionine β-synthase) inhibitor.
in vitro Aminooxyacetic acid (AOAA) is a malate-aspartate shuttle inhibitor, leading to decreased intracellular ATP levels and altering cell cycle and increasing apoptosis and necrosis of C6 glioma cells, without affecting the survival of primary astrocyte cultures. AOAA also decreases the glycolytic rate and the levels of extracellular lactate and pyruvate, without affecting the mitochondrial membrane potential of C6 cells. Aminooxyacetic acid can selectively inhibit the proliferation of breast cancer cells[1].
in vivo Aminooxyacetic acid suppresses the growth of breast adenocarcinoma in athymic mice[1]. AOAA is much less toxic in rats than in mice[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 C6 glioma cells
濃度 0.1, 0.25, 0.5, 1, 5 mM
反応時間 24 h
実験の流れ

C6 glioma cells are plated into 24-well or 12-well cell culture plates at the initial density of 1 × 106 cells/mL in Dulbecco's Modified Eagle's Medium containing 4500 mg/L D-glucose, 584 mg/L L-glutamine, and 1% penicillin and streptomycin, supplemented with 10% fetal bovine serum. The cells are maintained in a 5% CO2 incubator at 37℃. Cell survival is assessed 24 h after AOAA treatment by intracellular LDH assay.
動物実験 動物モデル female Wistar rats
投薬量 15 to 100 mg/kg
投与方法 i.p. or s.c.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

NRF2 activation by cysteine as a survival mechanism for triple-negative breast cancer cells [ Research Square, 2024, 10.21203/rs.3.rs-3849836/v1] PubMed: none
Dysregulated Gln-Glu-α-ketoglutarate axis impairs maternal decidualization and increases the risk of recurrent spontaneous miscarriage [ Cell Rep Med, 2023, 4(5):101026] PubMed: 37137303
Metabolic rewiring in keratinocytes by miR-31-5p identifies therapeutic intervention for psoriasis [ EMBO Mol Med, 2023, e15674.] PubMed: 36855912
The mitochondrial NADH shuttle system is a targetable vulnerability for Group 3 medulloblastoma in a hypoxic microenvironment [ Cell Death Dis, 2023, 14(11):784] PubMed: 38036520
L-cystathionine protects against oxidative stress and DNA damage induced by oxidized low-density lipoprotein in THP-1-derived macrophages [ Front Pharmacol, 2023, 14:1161542] PubMed: 37560474
Glutamine Metabolism Is Required for Alveolar Regeneration during Lung Injury [ Biomolecules, 2022, 12(5)728] PubMed: 35625656
Glutamate dehydrogenase 1 mediated glutaminolysis sustains HCC cells survival under glucose deprivation [ J Cancer, 2022, 13(3):1061-1072] PubMed: 35154470
Yap-Myc signaling induces pancreatic stellate cell activation through regulating glutaminolysis [ Exp Cell Res, 2022, 411(1):113000] PubMed: 34958764
Mistimed H 2 S Upregulation, Nrf2 Activation and Antioxidant Proteins Levels in Renal Tubular Epithelial Cells Subjected to Anoxia and Reoxygenation [ Biomed Rep, 2020, 13(2):3] PubMed: 32509306
Glutamate dehydrogenase 1 mediated glutaminolysis sustains HCC cells proliferation and survival under glucose deprivation [ Research Square, 2020, 10.21203/rs.3.rs-105979/v1] PubMed: None

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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