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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C284H432N84O79S7 |
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分子量 | 6511.51 | CAS No. | 9087-70-1 | |
Solubility (25°C)* | 体外 | Water | 100 mg/mL (15.35 mM) | |
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Aprotinin is a small protein serine protease inhibitor, used to reduce perioperative blood loss and transfusion. |
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in vitro | Aprotinin is an antifibrinolytic molecule that inhibits trypsin and related proteolytic enzymes. In cell biology, aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues. In the presence of aprotinin, the fibrinolytic activity is inhibited concentration dependently and the coagulation time is prolonged. Aprotinin is an effective inhibitor of the contact (intrinsic) coagulation pathway in vitro[2]. |
in vivo | Aprotinin inhibits clot lysis in vitro as well as rat-tail bleeding time in vivo and prolongs coagulation time in human plasma. In a rat arteriovenous shunt model, aprotinin reduces thrombus weight [2]. |
細胞アッセイ | 細胞株 | mouse G8-1 and C2C12 skeletal muscle myoblasts |
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濃度 | 2 TIU/ml | |
反応時間 | 12hrs | |
実験の流れ | Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days. |
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動物実験 | 動物モデル | Male Wistar rats |
投薬量 | 1.5 mg/kg and 3 mg/kg/h, 3 mg/kg and 6 mg/kg/h up to 5 mg/kg and 10 mg/kg/h | |
投与方法 | bolus injection followed by a maintenance infusion(i.p.) |
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SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation [ Elife, 2021, 10e65962] | PubMed: 33890572 |
Acetylation targets HSD17B4 for degradation via the CMA pathway in response to estrone. [ Autophagy, 2017, 13(3):538-553] | PubMed: 28296597 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。