ATP disodium

製品コードS1985 バッチS198501

印刷

化学情報

Chemical Structure Synonyms Adenosine-Triphosphate disodium, Adenosine 5'-triphosphate Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C10H14N5O13P3.2Na
分子量 551.14 CAS No. 987-65-5
Solubility (25°C)* 体外 Water 110 mg/mL (199.58 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 ATP disodium is a disodium salt form of adenosine-triphosphate which is a multifunctional nucleoside triphosphate.
in vitro

ATP release and autocrine feedback through P2Y2 and A3 receptors provide signal amplification, controlling gradient sensing and migration of neutrophils. [1] ATP results in production of reactive oxygen species (ROS), which stimulates the phosphatidylinositol 3-kinase (PI3K) pathway and subsequent Akt and ERK1/2 activation. ATP-dependent ROS production and PI3K activation also stimulate transcription of genes required for an oxidative stress response. ATP-mediated ROS-dependent PI3K is required for activation of caspase-1 and secretion of IL-1beta and IL-18. [2] ATP potently stimulates TNF-alpha release, resulting from TNF-alpha mRNA expression in rat cultured brain microglia. ATP-induced TNF-alpha release is Ca(2+)-dependent, and a sustained Ca(2+) influx correlated with the TNF-alpha release in ATP-stimulated microglia. ATP-induced TNF-alpha release is inhibited by PD 098059, an inhibitor of extracellular signal-regulated protein kinase (ERK) kinase 1 (MEK1), which activates ERK, and also by SB 203580, an inhibitor of p38 mitogen-activated protein kinase. ATP rapidly activates both ERK and p38 even in the absence of extracellular Ca(2+). [3] ATP-induced cytotoxicity is mediated by classical alterations of apoptosis, including membrane blebbing, nuclear condensation, and DNA fragmentation. ATP but not other nucleotides lead to the potent and selective activation of NF-κB in microglial cells through a P2Z receptor-mediated pathway. [4]
in vivo

ATP regulates microglial branch dynamics in the intact mice brain, and its release from the damaged tissue and surrounding astrocytes mediates a rapid microglial response towards injury. [5]

プロトコル(参考用のみ)

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure [ EBioMedicine, 2023, 10.1016/j.ebiom.2023.104863] PubMed: 37950995
Induction of Synthetic Lethality by Activation of Mitochondrial ClpP and Inhibition of HDAC1/2 in Glioblastoma [ Clin Cancer Res, 2022, 28(9):1881-1895] PubMed: 35417530
ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury [ Cell Signal, 2022, 93:110304] PubMed: 35278669
Extracellular phosphoprotein regulation is affected by culture system scale-down [ Biochim Biophys Acta Gen Subj, 2022, 1866(8):130165] PubMed: 35513203
Derivation and Characterization of Murine and Amphibian Müller Glia Cell Lines [ Transl Vis Sci Technol, 2022, 11(4):4] PubMed: 35377941
Human TBK1 deficiency leads to autoinflammation driven by TNF-induced cell death [ Cell, 2021, S0092-8674(21)00885-0] PubMed: 34363755
A Semi-automated System of Luciferase Immunoprecipitation Assay for Rapid and Easy Detection of African Swine Fever Virus Antibody [ J Clin Microbiol, 2021, JCM0099021] PubMed: 34260273
Dexmedetomidine Mitigated NLRP3-Mediated Neuroinflammation via the Ubiquitin-Autophagy Pathway to Improve Perioperative Neurocognitive Disorder in Mice [ Front Pharmacol, 2021, 12:646265] PubMed: 34079457
Fos Facilitates Gallid Alpha-Herpesvirus 1 Infection by Transcriptional Control of Host Metabolic Genes and Viral Immediate Early Gene [ Viruses, 2021, 13(6)1110] PubMed: 34207926
GSDMD membrane pore is critical for IL-1β release and antagonizing IL-1β by hepatocyte-specific nanobiologics is a promising therapeutics for murine alcoholic steatohepatitis. [ Biomaterials, 2020, 227:119570] PubMed: 31670032

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。