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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C15H18O2 |
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分子量 | 230.30 | CAS No. | 73069-13-3 | |
Solubility (25°C)* | 体外 | DMSO | 46 mg/mL warmed with 50ºC water bath (199.73 mM) | |
Ethanol | 46 mg/mL warmed with 50ºC water bath (199.73 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Atractylenolide I is the major sesquiterpenoid of the rhizome of A. macrocephala and shows a wide spectrum of pharmacological activities such as antiinflammatory, digestion promoting, and antioxidant effects. |
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in vitro | Atractylenolide I decreases the TNF-α level in LPS-stimulated peritoneal macrophages in a dosedependent manner, IC50=23.1 μM. It also inhibits NO production in LPS-activated peritoneal macrophages, IC50=41 μM. The IC50 of Atractylenolide I for inhibiting the activity of iNOS is 67.3 μM. It shows no marked effects against 5-lipoxygenase (5-LOX) and cyclooxygenase (COX). Atractylenolide I is also reported previously to act on white blood cell membranes and TLR4 and its antiinflammatory activity is related to antagonizing TLR4 [1]. |
in vivo | Atractylenolide I has anti-inflammatory effects. The treatment of Atractylenolide I significantly attenuates LPS-induced lung wet-to-dry weight ratio and MPO activity in LPS-induced acute lung injury mouse. It also significantly inhibits the production of TNF-α, IL-6, IL-1β, IL-13, and MIF production in bronchoalveolar lavage fluid (BALF), as well as neutrophils and macrophages in BALF, upregulates the production of IL-10 in BALF. Atractylenolide I protects mice acute lung injury induced by LPS via inhibition of TLR4 expression and NF-κB activation[2]. |
細胞アッセイ | 細胞株 | peritoneal macrophages |
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濃度 | 1-1000 μM | |
反応時間 | 24 h | |
実験の流れ | 2 × 104 cells per well are plated into 96-well plates, cells/well to a final volume of 200 μL, incubated at 37 °C for 24 h, and given a fresh change of medium. The cells are treated with atractylenolide I (1-1000 μM) dissolved in DMSO, and with LPS (final concentration 10 μg/mL) alone or in combination with atractylenolide I (1–100 μM) dissolved in DMSO. This DMSO percentage allows the optimal solubilization of atractylenolide I in aqueous solution. The control and LPS wells receive the same amount of DMSO. After 24 h of incubation at 37 °C, 20 μL of MTT (5 mg/mL) is added to each well and 4 h later the cells are lysed with 150 μL DMSO. The plate is shaken for 10 min and 30 min later the optical densities (OD570) for atractylenolide I are compared with the OD of the control or LPS stimulated wells to assess the cytotoxicity. | |
動物実験 | 動物モデル | LPS-induced acute lung injury mouse |
投薬量 | 5, 10 and 20 mg/kg | |
投与方法 | i.p. |
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Atractylenolide-I Sensitizes Triple-Negative Breast Cancer Cells to Paclitaxel by Blocking CTGF Expression and Fibroblast Activation [ Front Oncol, 2021, 11:738534] | PubMed: 34692516 |
Atractylenolide‑1 alleviates gastroparesis in diabetic rats by activating the stem cell factor/c‑kit signaling pathway [ Mol Med Rep, 2021, 24(4)691] | PubMed: 34368880 |
Atractylenolide I Induces Apoptosis and Suppresses Glycolysis by Blocking the JAK2/STAT3 Signaling Pathway in Colorectal Cancer Cells. [ Front Pharmacol, 2020, 26;11:273] | PubMed: 32273843 |
Lactobacillus Protects Against S. Typhimurium-Induced Intestinal Inflammation by Determining the Fate of Epithelial Proliferation and Differentiation. [ Mol Nutr Food Res, 2020, 64(5):e1900655] | PubMed: 31953989 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。