Baclofen

製品コードS4840 バッチS484001

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C10H12ClNO2
分子量 213.66 CAS No. 1134-47-0
Solubility (25°C)* 体外 0.01M HCl 3 mg/mL warmed with 50ºC water bath (14.04 mM)
Water 0.1 mg/mL (0.46 mM)
DMSO Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Baclofen is a gamma-amino-butyric acid (GABA) derivative used as a skeletal muscle relaxant and central nervous system depressant. It activates GABA receptors, specifically the GABAB receptors.
in vitro Baclofen induces G0/G1 phase arrest which is connected with down-regulation of intracellular cAMP level, and up-regulation of p21WAF1 protein expression as well as its phosphorylation level in human hepatocellular carcinoma cell[3].
in vivo Chronic treatment with baclofen markedly diminishes hippocampal atrophy and neuronal apoptosis in hippocampal CA1 area via the regulation of autophagy in chronic cerebral hypoperfusion in rats. Baclofen might suppress cytodestructive autophagic activity through Akt-GSK-3β-p-mTOR-Beclin 1 signaling pathway under chronic cerebral hypoperfusion. During brain ischemia, baclofen could reduce CX43 and CX36 surface expression. Baclofen-induced suppression of increased CX43 and CX36 surface expressions helps neurons survive[1]. Baclofen reduces reinstatement to cocaine self-administration in an animal model of relapse[2]. Baclofen suppresses Bel-7402 xenograft tumor growth in vivo[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Bel-7402, HuH-7, and HepG2 cells
濃度 25, 50 and 100 μM
反応時間 0-4 days
実験の流れ Bel-7402, HuH-7, and HepG2 cells are seeded into 96-well plates (10,000 per well) in a final volume of 200 μl medium. After a 24-hour attachment, cells are incubated with Bac (25, 50 and 100 μM) in the absence or presence of Pha (100 μM) for indicated time intervals. During this time, the culture medium is replaced with fresh medium every 24 h. After treatment, cell proliferation is evaluated by MTS assay. The OD values are measured at 490 nm with a microplate spectrophotometer.
動物実験 動物モデル male Sprague-Dawley rats of clean grade, aged 2-3 months, weighing 220-250 g
投薬量 12.5 mg/kg and 25 mg/kg
投与方法 i.p.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。