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Synonyms | Icariside II | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C27H30O10 |
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分子量 | 514.52 | CAS No. | 113558-15-9 | |
Solubility (25°C)* | 体外 | DMSO | 100 mg/mL (194.35 mM) | |
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Baohuoside I (Icariside II) is a flavonoid isolated from Epimedium koreanum Nakai with anti-inflammatory and anti-cancer activities. It may exert cytotoxic effect via the ROS/MAPK pathway. |
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in vitro | Baohuoside I accelerates apoptosis through the mitochondrial apoptotic pathway, involving the increment of BAX/Bcl-2 ratio, dissipation of mitochondrial membrane potential, transposition of cytochrome c, caspase 3 and caspase 9 activation, degradation of poly (ADP-ribose) polymerase and the over-production of reactive oxygen species (ROS)[1]. In vitro studies with three leukaemia and three solid tumour cell lines show that Baohuoside I has cytotoxic properties, with IC50 values of between 2.8 and 7.5 μg/ml[2]. |
in vivo | Baohuoside I is both anti-inflammatory and immunosuppressive, but unlike the cytotoxic and anti-rejection drugs it suppresses only B-cell (antibody) and not T-cell responses. Baohuoside I may have a role in therapy of those autoimmune diseases[2]. |
細胞アッセイ | 細胞株 | A549 cell |
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濃度 | 6.25, 12.5, and 25 μM | |
反応時間 | 24 h | |
実験の流れ | Cells (1×106 cells/ml) are exposed to Baohuoside I (6.25, 12.5, and 25 μM) for 24 h, washed with PBS, and then harvested with trypsin EDTA, counted, fixed with 75% ethanol and stored at 4℃ prior to DNA content analyses. Samples are resuspended in 1.0 ml hypotonic propidium iodide (PI)-solution (50 mg/ml PI, 0.1% sodium citrate, 0.1% Triton X-100, 0.1 mg/ml ribonuclease A) and incubated in the dark for 1 h. PI-stained samples are analyzed with a flow cytometer. | |
動物実験 | 動物モデル | Inbred female C57/BL/6J mice |
投薬量 | 20, 40, 60 mg/kg | |
投与方法 | i.p. |
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長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。