Batimastat (BB-94)

製品コードS7155 バッチS715501

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₃H₃₁N₃O₄S₂

分子量

477.64

CAS No.

130370-60-4

Solubility (25°C)*

体外

DMSO

96 mg/mL (200.98 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 Corn oil

1.25mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.
in vitro	Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. ^[1] Batimastat exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. ^[2]
in vivo	Batimastat can inhibit metastatic spread and growth of B16-BL6 murine melanoma. ^[1] In an orthotopic colon tumor model in mice, timastat treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).^[3] Batimastat reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. ^[4]

製品説明

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.

in vitro

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. ^[1]

Batimastat exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. ^[2]

in vivo

Batimastat can inhibit metastatic spread and growth of B16-BL6 murine melanoma. ^[1]

In an orthotopic colon tumor model in mice, timastat treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).^[3]

Batimastat reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. ^[4]

プロトコル(参考用のみ)

キナーゼアッセイ	Enzyme assays
キナーゼアッセイ	IC50s are determined for batimastat in vitro against different metalloproteinases in enzyme assays.
細胞アッセイ	細胞株	B16-BL6 cells
	濃度	3 nM (MMP-1), 4 nM (MMP-2 & MMP-9), 6 nM (MMP-7) and 20 nM (MMP-3), respectively
	反応時間	22 h
	実験の流れ	Cells were treated with different concentrations of batimastat was dissolved in absolute ethanol and were incubated for 22 hr and IC50 was measured.
動物実験	動物モデル	Nude mouse
	投薬量	30mg/ml
	投与方法	i.p.

参考

[4] Taraboletti G, et al. J Natl Cancer Inst, 1995, 87(4), 293-298.

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カスタマーフィードバック

: Data from [Data independently produced by , , Nature, 2017, 549(7673):533-537]

: Data from [Data independently produced by , , PLoS One, 2015, 10(5): e0127063]

: Data from [Data independently produced by , , Biomed Rep, 2016, 4(1):73-78. ]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions [ Adv Sci (Weinh), 2023, e2207084.]	PubMed: 36737855
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Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation [ Sci Adv, 2023, 9(7):eade8939]	PubMed: 36791204
Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells [ Nat Commun, 2022, 13(1):5494]	PubMed: 36123372
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Systematic analysis of the MAPK signaling network reveals MAP3K-driven control of cell fate [ Cell Syst, 2022, 13(11):885-894.e4]	PubMed: 36356576
Complete abrogation of key osteoclast markers with a membrane-anchored tissue inhibitor of metalloproteinase : a novel approach in the prevention of osteoclastogenesis [ Bone Joint Res, 2022, 11(11):763-776]	PubMed: 36331083
Aberrant NF-κB activation in odontoblasts orchestrates inflammatory matrix degradation and mineral resorption [ Int J Oral Sci, 2022, 14(1):6]	PubMed: 35082271
Bis[2-(Methacryloyloxy) Ethyl] Phosphate as a Primer for Enamel and Dentine [ J Dent Res, 2021, 220345211023477]	PubMed: 34238050
GSK-3α/β Activity Negatively Regulates MMP-1/9 Expression to Suppress Mycobacterium tuberculosis Infection [ Front Immunol, 2021, 12:752466]	PubMed: 35095838

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。