β-Glycerophosphate sodium salt hydrate

製品コードS3620 バッチS362004

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C3H9O6P.5H2O.2Na
分子量 306.11 CAS No. 13408-09-8
Solubility (25°C)* 体外 Water 61 mg/mL (199.27 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor.
in vitro β-glycerophosphate accelerates in vitro calcification by BVSMC and induces extensive calcium deposition in a manner analogous to in vitro mineralization by osteoblasts. β-glycerophosphate can increase ALP activity in osteoblasts[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 BVSMC (bovine vascular smooth muscle cells)
濃度 10 mmol/L
反応時間 14 d
実験の流れ BVSMC are cultured in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS (growing medium). After confluence, the cells are inoculated in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS in the presence of 10 mmol/L β-glycerophosphate, 10-7 mol/L insulin, and 50 μg/mL of ascorbic acid for 14 days (calcification medium). The medium is replaced with fresh medium every 3 days. In certain experiments, only β-glycerophosphate is used to induce BVSMC calcification. Moreover, in the time-course experiments, before being cultured in calcification medium (defined as day 0), cells are selected to be used as controls.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis [ Nat Commun, 2024, 15(1):2384] PubMed: 38493144
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1–mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644] PubMed: 36993303
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644] PubMed: 36993303
Sphingosine kinase 1 promotes tumor immune evasion by regulating the MTA3-PD-L1 axis [ Cell Mol Immunol, 2022, 19(10):1153-1167] PubMed: 36050478
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7] PubMed: 34997030
A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma [ Mol Med Rep, 2022, 26(2)255] PubMed: 35703357
MiR-140-5p upregulation suppressed β-glycerophosphate-induced vascular smooth muscle cell calcification via targeting TLR4 [ Immunopharmacol Immunotoxicol, 2022, 1-11] PubMed: 35272550
Identification of a Three-Glycolysis-Related lncRNA Signature Correlated With Prognosis and Metastasis in Clear Cell Renal Cell Carcinoma [ Front Med (Lausanne), 2021, 8:777507] PubMed: 35083240
ADORA1 Inhibition Promotes Tumor Immune Evasion by Regulating the ATF3-PD-L1 Axis [ Cancer Cell, 2020, 37(3):324-339.e8] PubMed: 32183950
ADORA1 Inhibition Promotes Tumor Immune Evasion by Regulating the ATF3-PD-L1 Axis. [ Cancer Cell, 2020, 37(3):324-339] PubMed: 32183950

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。