Blasticidin S HCl

製品コードS7419 バッチS741906

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C17H27ClN8O5
分子量 458.9 CAS No. 3513-03-9
Solubility (25°C)* 体外 Water 92 mg/mL (200.47 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes.
in vitro

Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. [1]

Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. [2]

Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. [3]

Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. [4]
in vivo

Blasticidin S HCl is an antibiotic isolated from Stretomyces girseochromogenes that acts as a protein synthesis inhibitor. It is used to select transfected cells carrying bsr or BSD resistance genes.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HEK293T cells
濃度 5 µg/ml
反応時間 7 days
実験の流れ

Lentivirus infected cells were maintained in 5 μg/ml blasticidin containing culture medium for 7d, with fresh medium exchange every day.
動物実験 動物モデル Mice
投薬量 2.5 mg/kg
投与方法 Oral

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

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USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
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Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin [ Mol Cell, 2023, 83(13):2206-2221.e11] PubMed: 37311463
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MERR APEX-seq protocol for profiling the subcellular nascent transcriptome in mammalian cells [ STAR Protoc, 2023, 4(1):102057] PubMed: 36853684
Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators [ Cell Rep Methods, 2023, 3(8):100544] PubMed: 37671014
A Nodal enhanced micropeptide NEMEP regulates glucose uptake during mesendoderm differentiation of embryonic stem cells [ Nat Commun, 2022, 13(1):3984] PubMed: 35810171

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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