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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C21H20N4O2 |
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分子量 | 360.41 | CAS No. | 896705-16-1 | ||||
Solubility (25°C)* | 体外 | DMSO | Insoluble | ||||
Water | Insoluble | ||||||
Ethanol | Insoluble | ||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | BMH-21 is a DNA intercalator, which binds ribosomal DNA and inhibits RNA polymerase I (Pol I) transcription. |
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in vitro | BMH-21 causes proteasome-dependent destruction of RPA194, the large catalytic subunit protein of Pol I holocomplex. [1] In U2OS cancer cell line, BMH-21 results in degradation of RPA194 and translocation of NCL with IC50 of 0.05 μM and 0.07 μM, respectively. By causing nucleolar stress, BMH-21 also shows potent inhibition on cell viability. [2] |
in vivo | BMH-21 reduces tumor growth in mouse xenografts. [1] |
細胞アッセイ | 細胞株 | U2OS osteosarcoma cells |
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濃度 | ~5 μM | |
反応時間 | 48 hours | |
実験の流れ | The cells are maintained at 37 °C in a humidified atmosphere containing 5% CO2. U2OS osteosarcoma cells are cultured in DMEM supplemented with 15% fetal bovine serum. Cells are plated in 96-well plates at a density of 10000 cells/well in triplicate and incubated for 48 h with the compounds. Viability is determined using WST-1 cell proliferation reagent. |
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動物実験 | 動物モデル | Athymic NCr nu/nu mice |
投薬量 | 25 or 50 mg/kg | |
投与方法 | i.p. |
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Data from [Data independently produced by , , Cell, 2018, 174(2):338-349]
Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression [ Elife, 2024, 13e80684] | PubMed: 38240312 |
CX‑5461 potentiates imatinib‑induced apoptosis in K562 cells by stimulating KIF1B expression [ Exp Ther Med, 2024, 27(3):107.] | PubMed: 38356673 |
CX‑5461 potentiates imatinib‑induced apoptosis in K562 cells by stimulating KIF1B expression [ Exp Ther Med, 2024, 27(3):107] | PubMed: 38356673 |
The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress [ bioRxiv, 2024, 10.1101/2023.12.29.573614] | PubMed: none |
The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress [ bioRxiv, 2024, 10.1101/2023.12.29.573614] | PubMed: none |
The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination [ Cell Discov, 2023, 9(1):99] | PubMed: 37789001 |
The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination [ Cell Discov, 2023, 9(1):99] | PubMed: 37789001 |
Ribosome biosynthesis and Hedgehog activity are cooperative actionable signaling mechanisms in breast cancer following radiotherapy [ NPJ Precis Oncol, 2023, 7(1):61] | PubMed: 37380890 |
Ribosome biosynthesis and Hedgehog activity are cooperative actionable signaling mechanisms in breast cancer following radiotherapy [ NPJ Precis Oncol, 2023, 7(1):61] | PubMed: 37380890 |
Glutamine deficiency in solid tumor cells confers resistance to ribosomal RNA synthesis inhibitors [ Nat Commun, 2022, 13(1):3706] | PubMed: 35764642 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。