BMH-21

製品コードS7718 バッチS771803

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C21H20N4O2
分子量 360.41 CAS No. 896705-16-1
Solubility (25°C)* 体外 DMSO 6 mg/mL warmed with 50ºC water bath (16.64 mM)
Ethanol 2 mg/mL warmed with 50ºC water bath (5.54 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 BMH-21 is a DNA intercalator, which binds ribosomal DNA and inhibits RNA polymerase I (Pol I) transcription.
in vitro

BMH-21 causes proteasome-dependent destruction of RPA194, the large catalytic subunit protein of Pol I holocomplex. [1]

In U2OS cancer cell line, BMH-21 results in degradation of RPA194 and translocation of NCL with IC50 of 0.05 μM and 0.07 μM, respectively. By causing nucleolar stress, BMH-21 also shows potent inhibition on cell viability. [2]
in vivo

BMH-21 reduces tumor growth in mouse xenografts. [1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 U2OS osteosarcoma cells
濃度 ~5 μM
反応時間 48 hours
実験の流れ

The cells are maintained at 37 °C in a humidified atmosphere containing 5% CO2. U2OS osteosarcoma cells are cultured in DMEM supplemented with 15% fetal bovine serum. Cells are plated in 96-well plates at a density of 10000 cells/well in triplicate and incubated for 48 h with the compounds. Viability is determined using WST-1 cell proliferation reagent.
動物実験 動物モデル Athymic NCr nu/nu mice
投薬量 25 or 50 mg/kg
投与方法 i.p.

カスタマーフィードバック

Data from [Data independently produced by , , Cell, 2018, 174(2):338-349]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression [ Elife, 2024, 13e80684] PubMed: 38240312
CX‑5461 potentiates imatinib‑induced apoptosis in K562 cells by stimulating KIF1B expression [ Exp Ther Med, 2024, 27(3):107.] PubMed: 38356673
CX‑5461 potentiates imatinib‑induced apoptosis in K562 cells by stimulating KIF1B expression [ Exp Ther Med, 2024, 27(3):107] PubMed: 38356673
The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress [ bioRxiv, 2024, 10.1101/2023.12.29.573614] PubMed: none
The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress [ bioRxiv, 2024, 10.1101/2023.12.29.573614] PubMed: none
The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination [ Cell Discov, 2023, 9(1):99] PubMed: 37789001
The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination [ Cell Discov, 2023, 9(1):99] PubMed: 37789001
Ribosome biosynthesis and Hedgehog activity are cooperative actionable signaling mechanisms in breast cancer following radiotherapy [ NPJ Precis Oncol, 2023, 7(1):61] PubMed: 37380890
Ribosome biosynthesis and Hedgehog activity are cooperative actionable signaling mechanisms in breast cancer following radiotherapy [ NPJ Precis Oncol, 2023, 7(1):61] PubMed: 37380890
Glutamine deficiency in solid tumor cells confers resistance to ribosomal RNA synthesis inhibitors [ Nat Commun, 2022, 13(1):3706] PubMed: 35764642

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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