BRD7552

製品コードS7363 バッチS736301

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₃₃H₃₃N₃O₁₅
	分子量	711.63	CAS No.	1137359-47-7
Solubility (25°C)*	体外	DMSO	100 mg/mL (140.52 mM)
		Ethanol	100 mg/mL (140.52 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	BRD7552 is an inducer of transcription factor PDX1, which increases insulin expression.
in vitro	BRD7552 upregulates PDX1 expression in both primary human islets and ductal cells, and induces epigenetic changes in the PDX1 promoter consistent with transcriptional activation. BRD7552 increases PDX1 mRNA levels by 2- to 4-fold, with a maximal effect at 5 μM and the expression of MAFA. BRD7552 increases PDX1 expression in mouse αTC cells but not βTC cells. Treatment of PANC-1 cells with BRD7552 causes a dose-dependent increase in insulin mRNA expression. ^[1]

製品説明

BRD7552 is an inducer of transcription factor PDX1, which increases insulin expression.

in vitro

BRD7552 upregulates PDX1 expression in both primary human islets and ductal cells, and induces epigenetic changes in the PDX1 promoter consistent with transcriptional activation. BRD7552 increases PDX1 mRNA levels by 2- to 4-fold, with a maximal effect at 5 μM and the expression of MAFA. BRD7552 increases PDX1 expression in mouse αTC cells but not βTC cells. Treatment of PANC-1 cells with BRD7552 causes a dose-dependent increase in insulin mRNA expression. ^[1]

プロトコル(参考用のみ)

キナーゼアッセイ	ELISA
キナーゼアッセイ	The Human PDX1 ELISA Kit is used to quantitatively determine PDX1 concentrations in cell lysates. Brieﬂy, 100 μL of standards or samples are added into a 96-well and incubated for 2 h at 37℃. Liquid from each well is removed, and wells are incubated for 1 h with biotinylated anti-body, washed 3 times, and incubated with horseradish peroxidase-avidin. 3,3’,5,5’-Tetramethylbenzidine substrate is added to each well and incubated for 15-30 min at 37℃ followed by stop solution. The optical density of each well is determined within 5 min using a microplate reader set to 450 nm.

参考

[1] Yuan Y, et al. Chem Biol. 2013, 20(12), 1513-1522.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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