BrdU (Bromodeoxyuridine)

製品コードS7918 バッチS791806

印刷

化学情報

Synonyms

5-Bromo-2'-deoxyuridine, BUdR

Storage
(From the date of receipt)

3 years -20°C(in the dark) powder

化学式

C₉H₁₁BrN₂O₅

分子量

307.1

CAS No.

59-14-3

Solubility (25°C)*

体外

DMSO

61 mg/mL (198.63 mM)

Ethanol (warmed with 50ºC water bath)

3 mg/mL (9.76 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

4%DMSO 1 Corn oil

7.5mg/ml

Taking the 1 mL working solution as an example, add 40 μL of 187.5 mg/ml clear DMSO stock solution to 960 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	BrdU (Bromodeoxyuridine) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.
in vitro	In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. ^[1] BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. ^[2]
in vivo	In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. ^[1]

製品説明

BrdU (Bromodeoxyuridine) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.

in vitro

In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. ^[1]

BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. ^[2]

in vivo

In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. ^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	RG2 rat glioma cells
	濃度	~ 50 µM
	反応時間	24 hours
	実験の流れ	Cultures are initially plated at 2000 cells/cm² and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves were obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.
動物実験	動物モデル	Rats bearing RG2 tumors
	投薬量	300 mg/kg (i.p.); 0.8 mg/ml (p.o.)
	投与方法	i.p. or p.o.

参考

カスタマーフィードバック

: Data from [Data independently produced by , , PLoS One, 2016, 11(11):e0166386.]

: Data from [Data independently produced by , , PLoS One, 2016, 11(11):e0166386]

: Data from [Data independently produced by , , Int J Ophthalmol, 2017, 10(4):507-514]

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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