Catalpol

製品コードS3909 バッチS390903

印刷

化学情報

Synonyms

Catalpinoside, Digitalis purpurea L

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₅H₂₂O₁₀

分子量

362.33

CAS No.

2415-24-9

Solubility (25°C)*

体外

DMSO

72 mg/mL (198.71 mM)

Water

72 mg/mL (198.71 mM)

Ethanol

4 mg/mL (11.03 mM)

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.6mg/ml	Taking the 1 mL working solution as an example, add 50 μL of clarified DMSO stock solution of 72 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% corn oil	3.6mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 72 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Catalpol (Catalpinoside, Digitalis purpurea L), an iridoid glucoside contained richly in the roots of the small flowering plant species Rehmannia glutinosa Libosch, has been shown to have antioxidation, anti-inflammation, anti-apoptosis and other neuroprotective properties and plays a role in neuroprotection against hypoxic/ischemic injury, AD and PD in both in vivo and in vitro models.
in vitro	Catalpol is able to inhibit HCT116 cell proliferation. Furthermore, catalpol induces apoptosis in HCT116 cells, which depends on the increased activities of caspase-3 and −9. In addition, catalpol reduces the expression of PI3K, p-Akt and Akt in HCT116 cells^[1]. In vitro, catalpol inhibits TNF-α, IL-6, IL-4 and IL-1β production and up-regulates IL-10 expression in LPS-stimulated alveolar macrophages^[2].
in vivo	Catalpol decreases peroxynitrite formation and consequently exerts cardioprotective effects through the PI3K/Akt signaling pathway in ischemic/reperfusion rats. Catalpol inhibits lung W/D ratio, myeloperoxidase activity of lung samples, the amounts of inflammatory cells and TNF-α, IL-6, IL-4 and IL-1β in BALF induced by LPS. The production of IL-10 in BALF is up-regulated by catalpol^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HCT116 cells
	濃度	0, 25, 50 and 100 µg/ml
	反応時間	24, 48, 72 h
	実験の流れ	HCT116 cells (2×10⁴ cells/well) are seeded in 96-well plates and cell viability is detected using MTT. HCT116 cells are cultured with various concentrations of catalpol (0, 25, 50 and 100 µg/ml). Following treatment for 24, 48, and 72 h, 20 µl MTT solution (0.5 mg/ml) is added into each well and cells are incubated at 37°C for 4 h. The culture medium of each well is subsequently removed and 150 µl dimethyl sulfoxide is added into each well at room temperature whilst being shaken for 20 min. Absorbance is measured at 570 nm using a Bio-Rad ELISA reader.
動物実験	動物モデル	Mice acute lung injury model (Male BALB/c mice)
	投薬量	2.5, 5 and 10 mg/kg
	投与方法	i.p.

参考

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。