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Synonyms | Ciclopirox olamine, HOE 296,Ciclopiroxolamine | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C12H17NO2.C2H7NO |
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分子量 | 268.35 | CAS No. | 41621-49-2 | ||||
Solubility (25°C)* | 体外 | DMSO | 40 mg/mL (149.05 mM) | ||||
Ethanol | 30 mg/mL (111.79 mM) | ||||||
Water | Insoluble | ||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Ciclopirox ethanolamine (Ciclopirox olamine, HOE 296,Ciclopiroxolamine) is a broad-spectrum antifungal agent working as an iron chelator. |
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in vitro | Ciclopirox significantly inhibits the growth of C. albicans strain SC5314 cells, with MIC80s of 1.0-2.0 μg/mL, growth decreased dramatically at the concentrations of >0.6 μg/mL, and almost complete growth inhibition at concentration of 0.7 μg/mL, unlike fluconazole which shows a much wider range of concentrations with intermediate inhibition. Like iron chelator bipyridine, Ciclopirox reduces cell growth by binding to iron ions, which can be reversed by addition of FeCl3. Moreover, Ciclopirox treatment at subinhibitory concentration (0.6 μg/mL) only moderately reduces the virulence genes such as genes encoding secreted proteinases or lipases, but leads to a distinct up- or down-regulation of genes encoding iron permeases or transporters (FTR1, FTR2, and FTH1), a copper permease (CCC2), an iron reductase (CFL1), and a siderophore transporter (SIT1). Although the Candida drug resistance genes CDR1 and CDR2 are up-regulated after Ciclopirox treatment, no change in resistance or increased tolerance could be observed even after an incubation period of 6 months, in contrast to fluconazole in which the MICs for cells noticeably increase after 2 months. [1] Ciclopirox inhibits the growth of Aspergillus fumigatus strain B5233 with IC50 of 4.22 μM, more potently compared with deferiprone with IC50 of 1.29 mM. [2] |
特徴 | Exhibits antifungal activity via a specific iron limiation mechanism, with no single case of fungal resistance reported. |
細胞アッセイ | 細胞株 | C. albicans strain SC5314 |
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濃度 | Dissolved in DMSO, final concentrations 10 μg/mL | |
反応時間 | 1-10 hours | |
実験の流れ | Sabouraud glucose medium (2%) is used for cell culture growth, and RPMI 2% glucose medium and 2% Sabouraud glucose medium are used for MIC determinations. For cell culture growth curves, 220 mL of 2% Sabouraud glucose medium containing different concentrations of Ciclopirox are inoculated with 105 cells/mL, and the mixture is shaken at 160 rpm and 37 °C for 1-10 hours. Growth is measured photometrically at 630 nm. FeCl3 or 2,2'-bipyridine is added to the medium at different concentrations for inhibition studie |
Inference of glioblastoma migration and proliferation rates using single time-point images [ Commun Biol, 2023, 6(1):402] | PubMed: 37055469 |
Iron-dependent epigenetic modulation promotes pathogenic T cell differentiation in lupus [ J Clin Invest, 2022, 132(9)e152345] | PubMed: 35499082 |
TRIM7 modulates NCOA4-mediated ferritinophagy and ferroptosis in glioblastoma cells [ Redox Biol, 2022, 56:102451] | PubMed: 36067704 |
Leveraging patient derived models of FGFR2 fusion positive intrahepatic cholangiocarcinoma to identify synergistic therapies [ NPJ Precis Oncol, 2022, 6(1):75] | PubMed: 36274097 |
MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1 [ Anal Cell Pathol (Amst), 2022, 2022:4213401] | PubMed: 35646516 |
Transcriptional Repression of Ferritin Light Chain Increases Ferroptosis Sensitivity in Lung Adenocarcinoma [ Front Cell Dev Biol, 2021, 9:719187] | PubMed: 34765600 |
Synthetic Derivatives of Ciclopirox are Effective Inhibitors of Cryptococcus neoformans [ ACS Omega, 2021, 6(12):8477-8487] | PubMed: 33817509 |
High-throughput functional annotation of natural products by integrated activity profiling [ bioRxiv, 2019, 10.1101/748129] | PubMed: N/A |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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