Coelenterazine

製品コードS7777 バッチS777701

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
化学式

C26H21N3O3
分子量 423.46 CAS No. 55779-48-1
Solubility (25°C)* 体外 DMSO 84 mg/mL (198.36 mM)
Ethanol 1 mg/mL (2.36 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine have been used as bioluminescence donors in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe, and it can also be used in the chemiluminescent detection of peroxynitrite.
in vitro

When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 礸/ml) shows a bioluminescence signal. [1] In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations. [2]
in vivo

In 4T1-implanted mouse, Coelenterazine detects cancer-associated superoxide anion and used to evaluate the role of superoxide anion in cancer cell biology and its role in pathology. [2]
密度 1.32 g/mL

プロトコル(参考用のみ)

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

RNAi-mediated rheostat for dynamic control of AAV-delivered transgenes [ Nat Commun, 2023, 14(1):1970] PubMed: 37031257
The molecular evolution of C4-associated gene regulation in the genus Flaveria [ The UWA Research Repository, 2023, ] PubMed: none
Exendin-4 gene modification and microscaffold encapsulation promote self-persistence and antidiabetic activity of MSCs [ Sci Adv, 2021, 7(27)eabi4379] PubMed: 34215590
Development of a Bioassay for Continuous Monitoring of TWIST1 Activity [ Preprints, 2021, 10.20944/preprints202103.0265.v1] PubMed: N/A
A novel bioassay for quantification of surface Cannabinoid receptor 1 expression [ Sci Rep, 2020, 10(1):18191] PubMed: 33097803
Tumor treating fields increases membrane permeability in glioblastoma cells [ Cell Death Discov, 2018, 4:113] PubMed: 30534421

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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