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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C32H46O8 |
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分子量 | 558.70 | CAS No. | 6199-67-3 | |
Solubility (25°C)* | 体外 | DMSO | 100 mg/mL (178.98 mM) | |
Ethanol | 50 mg/mL warmed with 50ºC water bath (89.49 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Cucurbitacin B, an oxygenated tetracyclic triterpenoid compound extracted from Cucurbitaceae plant species, is a long-term anticancer agent by disruption of microtubule polymerization. |
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in vitro | Cucurbitacin B inhibits the growth of 5 GBM cell lines: U87, U118, U343, U373 and T98G by affecting the cytoskeleton and the JNK pathway, with ED50 ranged from 5 nM to 100 nM. Cucurbitacin B also causes cell cycle arrest at the G2/M phase, decreases cell migration and induces apoptosis of GBM cells. [1] In human breast cancer cell lines, Cucurbitacin B also inhibits cell growth with ED50 varied from 30.3 nM to 418 nM, and rapidly induces cytoskeletal disruption. [2] In addition, Cucurbitacin B possesses immunomodulatory and anti-inflammatory effects, depending on the induction of heme oxygenase-1 expression via Nrf2 activation. [3] |
in vivo | In nude mice bearing MDA-MB-231 tumors, Cucurbitacin B (1 mg/kg, i.p.) inhibits tumor growth by 55 %, and causes lower serum glucose levels. [2] |
細胞アッセイ | 細胞株 | U87, T98G, U118, U343, and U373 cells |
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濃度 | ~1 μM | |
反応時間 | 48 h | |
実験の流れ | For proliferation measurements, the cells are placed into 96 well plates, and cell growth is measured at various times by MTT assay according to the protocol. | |
動物実験 | 動物モデル | Female nude mice bearing MDA-MB-231 tumors |
投薬量 | 1 mg/kg | |
投与方法 | i.p. |
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NLRP3 inflammasome inhibitor cucurbitacin B suppresses gout arthritis in mice [ J Mol Endocrinol, 2021, 67(2):27-40] | PubMed: 34047713 |
Targeting NAD+ biosynthesis overcomes panobinostat and bortezomib-induced malignant glioma resistance. [ Mol Cancer Res, 2020, 10.1158/1541-7786.MCR-19-0669] | PubMed: 32238439 |
Cucurbitacin B inhibits cell proliferation and induces cell apoptosis in colorectal cancer by modulating methylation status of BTG3 [ Neoplasma, 2019, 66(4):593-602] | PubMed: 31058532 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。