Curcumin analog C1

製品コードS6769 バッチS676901

印刷

化学情報

Synonyms

Curcumin analog Compound C1, TFEB activator 1

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₉H₁₈O₃

分子量

294.34

CAS No.

39777-61-2

Solubility (25°C)*

体外

DMSO

30 mg/mL (101.92 mM)

Ethanol

2 mg/mL (6.79 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

1.5mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Curcumin analog C1 (Curcumin analog Compound C1) is a potent transcription factor EB (TFEB) activator with promise for the prevention or treatment of Alzheimer's disease.
in vitro	Curcumin analog C1 specifically binds to TFEB at the N terminus and promotes TFEB nuclear translocation without inhibiting MTOR activity. By activating TFEB, Curcumin analog C1 enhances autophagy and lysosome biogenesis in N2a cells. Curcumin analog C1 (0.2-1 μM) dose-dependently increases the levels of LC3-II and SQSTM1/p62 in N2a cells.^[2]
in vivo	The LD⁵⁰ value of Curcumin analog C1 is 175 mg/kg in the acute toxicity assay. Short-term oral administration of Curcumin analog C1 (low dosage 10 mg/kg and high dosage 25 mg/kg; for 24 h) dose-dependently increases the expression of LC3B-II and TFEB in the liver, frontal cortex and striatum of the brain. Chronic administration of Curcumin analog C1 (10 mg/kg; daily; for 21 days) activates TFEB and enhances autophagy in rat brains. ^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	N2a cells, HeLa cells
	濃度	0.2 μM-1 μM
	反応時間	12 h
	実験の流れ	N2a cells are treated with curcumin (Cur, 10 μM) and Curcumin analog C1 (1 μM) for 12 h. Cells are fixed and stained with TFEB antibody and DAPI for showing the nuclear translocation of endogenous TFEB. N2a cells are incubated with Curcumin analog C1 (1 μM) for 12 h. The protein levels of LC3B-II and SQSTM1/p62 are determined by western blotting. N2a and HeLa cells are treated with Curcumin analog C1 (0.5, 1 μM) or sucrose (100 mM) for 12 h. The protein levels of the autophagy marker (LC3B-II), TFEB, and lysosome markers (LAMP1, CTSD) are determined by western blotting.
動物実験	動物モデル	Adult male Sprague-Dawley (SD) rats weighing 350 to 400 g
	投薬量	10 mg/kg, 25 mg/kg
	投与方法	o.g.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Functional TFEB activation characterizes multiple models of renal cystic disease and loss of polycystin-1 [ Am J Physiol Renal Physiol, 2023, 324(4):F404-F422]	PubMed: 36794754
TFEB-Mediated Lysosomal Restoration Alleviates High Glucose-Induced Cataracts Via Attenuating Oxidative Stress [ Invest Ophthalmol Vis Sci, 2022, 63(6):26]	PubMed: 35758908

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。