Deoxyarbutin

製品コードS4132 バッチS413201

印刷

化学情報

Chemical Structure Synonyms D-arbutin,D-Arb Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C11H14O3
分子量 194.23 CAS No. 53936-56-4
Solubility (25°C)* 体外 DMSO 39 mg/mL (200.79 mM)
Ethanol 39 mg/mL (200.79 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 DeoxyArbutin(D-arbutin,D-Arb) is a reversible tyrosinase inhibitor, inhibiting tyrosinase activity with IC50 of 50 nM.
in vitro

DeoxyArbutin demonstrates effective inhibition of mushroom tyrosinase in vitro with a Ki that is 10-fold lower that hydroquinone (HQ) and 350-fold lower than arbutin. [1] DeoxyArbutin exhibits toxicity in darkly (higher tyrosinase activity) and lightly (lower tyrosinase activity) pigmented melanocytes with IC50 of 57.5 μM and 104.7 μM. DeoxyArbutin (100 μM) dose-dependently inhibits tyrosine hydroxylase activity in lysate from untreated, darkly pigmented melanocytes. DeoxyArbutin (100 μM) inhibits tyrosine hydroxylase activity of mushroom tyrosinase in a dose-dependent manner. DeoxyArbutin inhibits tyrosine hydroxylase activity of mushroom tyrosinase with IC50 of 17.5 μM and Ki of 21.6 μM. [2] Water increases the decomposition of DeoxyArbutin in the formulations and the polyol-in-silicone, oil-based, anhydrous emulsion system provides a relatively stable surrounding for the DeoxyArbutin that delays its degradation at 25 ℃ and 45 ℃. [3] DeoxyArbutin inhibits melanin synthesis in intact melanocytes in a dose-dependent manner. DeoxyArbutin exhibits dose-dependent reductions in melanocyte cell number, primarily due to inhibition of proliferation rather than initiation of apoptosis. DeoxyArbutin increases minimal amounts of reactive oxygen species (ROS) with subsequently induced expression of the endogenous antioxidant catalase in treated melanocytes.[4]
in vivo

DeoxyArbutin (3%) demonstrates rapid and sustained skin lightening that is completely reversible within 8 weeks after halt in topical application in a hairless, pigmented guinea pig model. [1]
特徴 More effective than hydroquinone in inhibiting mushroom tyrosinase.

プロトコル(参考用のみ)

キナーゼアッセイ Tyrosinase inhibition assay
Briefly, 20 mL of mushroom tyrosinase (3.2 mg) in 3-(N-Morpholino)-propanesulfonic acid buffer (100 mM, pH 7.0) is added to 10 mL of 0.5 mM DOPA and incubated for 10 min at 70℃. About 100 mL of test inhibitor (0.1 mM-10.0 mM) plus 200 mL of tyrosine (10 mM) are added and incubated for an additional 22 min. The reaction solution is cooled to room temperature and used for HPLC quantification of tyrosine consumption (mM tyrosine/min/mg tyrosinase). About 6 min, run times of a 5 mL sample through a HPLC Supelcosil LC-CN column (25 cm?.6 mm) using a 470 scanning fluorescence detector are employed to allow separation of tyrosine from DOPA and inhibitor. The mobile phase used is 0.05 M acetic acid/20 mM triethylamine. Units of inhibition represent percent activity of sample treated with inhibitor compare to sample treated with vehicle only.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。