Deoxycholic acid

製品コードS4689 バッチS468901

印刷

化学情報

Chemical Structure Synonyms Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C24H40O4
分子量 392.57 CAS No. 83-44-3
Solubility (25°C)* 体外 DMSO 78 mg/mL (198.69 mM)
Ethanol 60 mg/mL (152.83 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO Corn oil
3.9mg/ml Taking the 1 mL working solution as an example, add 50 μL of 78 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of deoxycholic acid is by means of decreased cell membrane integrity.
in vitro Deoxycholic acid inhibits miR-21 expression in primary rat hepatocytes in a dose-dependent manner, and increases miR-21 pro-apoptotic target programmed cell death 4 (PDCD4) and apoptosis. Deoxycholic acid decreases NF-κB activity, shown to represent an upstream mechanism leading to modulation of the miR-21/PDCD4 pathway[1].
in vivo miR-21 expression is down-regulated upon short exposures to Deoxycholic acid, as is the downstream pathway, with concomitant PIDD processing and activation of caspase-2, which contributes to DCA-induced liver damage[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Primary rat hepatocytes
濃度 25 to 200 μM
反応時間 24 h
実験の流れ

Primary rat hepatocytes were isolated from male rats (100 to 150 g) by collagenase perfusion. After isolation, hepatocytes were resuspended in complete Williams E medium and plated on BD Primaria™ culture dishes at 5 × 104 cells/cm2. Cells are kept at 37 °C in a humidified atmosphere of 5% CO2 for 4-6 h to allow attachment. Plates are then washed with phosphate buffered saline (PBS) 1× in order to remove dead cells and incubated in Williams E medium supplemented with 25 to 200 μM DCA or no addition (control) for 24 h. Primary rat hepatocytes are processed for total RNA and protein isolation, cell viability, cytotoxicity and caspase activity assays and Hoechst staining.
動物実験 動物モデル Male Wistar rats
投薬量 125 mg/kg
投与方法 p.o.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Analysis of the Ubiquitination and Phosphorylation of Vangl Proteins [ Bio Protoc, 2022, 12(20)e4533] PubMed: 36353719

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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