Dibutyryl-cAMP (Bucladesine)

製品コードS7858 バッチS785811

印刷

化学情報

Chemical Structure Synonyms dbcAMP Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C18H23N5NaO8P
分子量 491.37 CAS No. 16980-89-5
Solubility (25°C)* 体外 DMSO (warmed with 50ºC water bath) 98 mg/mL (199.44 mM)
Water (warmed with 50ºC water bath) 98 mg/mL (199.44 mM)
Ethanol (warmed with 50ºC water bath) 25 mg/mL (50.87 mM)
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.9mg/ml Taking the 1 mL working solution as an example, add 50 μL of 98 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Dibutyryl-cAMP (Bucladesine, dbcAMP,Dibutyryl cAMP sodium salt) is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.
in vitro

Dibutyryl-cAMP inhibits neuronal glucose uptake via PKA activation. [1]

In cultured rat hepatocytes, Dibutyryl-cAMP inhibits inducible nitric oxide synthase expression and NF-kappaB-binding activity. [2]

Dibutyryl-cAMP also suppress TNFalpha-induced hepatocyte apoptosis by inhibiting FADD up-regulation. [3]
in vivo

In a mouse model, bucladesine (600 nM/mouse, i.p.) reverses zinc chloride- and lead acetate-induced avoidance memory retention impairments. [4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 PC12 cells
濃度 1 mM
反応時間 24 h
実験の流れ

Cells were incubated with 1 mM of db-cAMP.
動物実験 動物モデル Mice
投薬量 600 nM/mouse
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Activation of hepatic adenosine A1 receptor ameliorates MASH via inhibiting SREBPs maturation [ Cell Rep Med, 2024, 5(3):101477] PubMed: 38508143
Jiao-tai-wan and its effective component-berberine improve diabetes and depressive disorder through the cAMP/PKA/CREB signaling pathway [ J Ethnopharmacol, 2024, 324:117829] PubMed: 38296172
IL-6-Derived Autocrine Lactate Promotes Immune Escape of Uveal Melanoma [ Invest Ophthalmol Vis Sci, 2024, 65(3):37] PubMed: 38551584
Host-to-graft propagation of inoculated α-synuclein into transplanted human induced pluripotent stem cell-derived midbrain dopaminergic neurons [ Regen Ther, 2024, 25:229-237] PubMed: 38283940
Axonal Lysosomal Assays for Characterizing the Effects of LRRK2 G2019S [ Biology (Basel), 2024, 13(1)58] PubMed: 38275734
Balanced SET levels favor the correct enhancer repertoire during cell fate acquisition [ Nat Commun, 2023, 14(1):3212] PubMed: 37270547
Probabilistic cell seeding and non-autofluorescent 3D-printed structures as scalable approach for multi-level co-culture modeling [ Mater Today Bio, 2023, 21:100706] PubMed: 37435551
Elevated high-mannose N-glycans hamper endometrial decidualization [ iScience, 2023, 10.1016/j.isci.2023.108170] PubMed: 37915610
Expression and role of nicotinic acetylcholine receptors during midbrain dopaminergic neuron differentiation from human induced pluripotent stem cells [ Neuropsychopharmacol Rep, 2023, 10.1002/npr2.12361] PubMed: 37366076
Persistent DNA damage associated with ATM kinase deficiency promotes microglial dysfunction [ Nucleic Acids Res, 2022, 50(5):2700-2718] PubMed: 35212385

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。