DPI (Diphenyleneiodonium chloride)

製品コードS8639 バッチS863902

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₂H₈I.Cl

分子量

314.55

CAS No.

4673-26-1

Solubility (25°C)*

体外

DMSO

18 mg/mL (57.22 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

3%DMSO 1 40%PEG300 2 2%Tween80 3 55%ddH2O

0.5mg/ml

Taking the 1 mL working solution as an example, add 30 μL of clarified DMSO stock solution of 16.6666666666667 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 20 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 550 μL of ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	DPI (Diphenyleneiodonium chloride) is an inhibitor of NADPH oxidase and also a potent, irreversible, and time-, temperature-dependent iNOS/eNOS inhibitor. Diphenyleneiodonium chloride (DPI) also functions as a TRPA1 activator and selectively inhibits intracellular reactive oxygen species (ROS).
in vitro	DPI inhibits the activity of NADPH oxidase, nitric oxide synthase, xanthine oxidase and NADPH cytochrome P450 oxidoreductase^[4]. Femtomolar concentrations of DPI exert potent anti-inflammatory and neuroprotective effects by inhibiting microglial activation through the inhibition of ERK-regulated PHOX activity^[1]. DPI has frequently been used to inhibit ROS production mediated by various flavoenzymes, including NAD(P)H oxidase, quinone oxidoreductase, cytochrome P450 reductase and nitric oxide synthase^[2]. NADPH, NADP+, and 2'5'-ADP blocks the inhibitory action of DPI^[3]. DPI treatment in ARPE-19 cells evoked a dose- and time-dependent growth inhibition, and also induced DNA fragmentation and protein content of the proapoptotic factor Bax. In addition, DPI signiﬁcantly induced the expression and phosphorylation of p53, which induces proapoptotic genes in response to DNA damage or irreparable cell cycle arrest. ROS have been implicated as a key factor in the activation of p53 by many chemotherapeutic drugs^[4].
in vivo	Diphenyleneiodonium (DPI), an NADPH oxidase inhibitor, inhibited the production of pro-inflammatory cytokines, (TNF-α and IL-6), reduced macrophage infiltration and classical polarization, and induced the ROS generation.

製品説明

DPI (Diphenyleneiodonium chloride) is an inhibitor of NADPH oxidase and also a potent, irreversible, and time-, temperature-dependent iNOS/eNOS inhibitor. Diphenyleneiodonium chloride (DPI) also functions as a TRPA1 activator and selectively inhibits intracellular reactive oxygen species (ROS).

in vitro

DPI inhibits the activity of NADPH oxidase, nitric oxide synthase, xanthine oxidase and NADPH cytochrome P450 oxidoreductase^[4].

Femtomolar concentrations of DPI exert potent anti-inflammatory and neuroprotective effects by inhibiting microglial activation through the inhibition of ERK-regulated PHOX activity^[1].

DPI has frequently been used to inhibit ROS production mediated by various flavoenzymes, including NAD(P)H oxidase, quinone oxidoreductase, cytochrome P450 reductase and nitric oxide synthase^[2].

NADPH, NADP+, and 2'5'-ADP blocks the inhibitory action of DPI^[3].

DPI treatment in ARPE-19 cells evoked a dose- and time-dependent growth inhibition, and also induced DNA fragmentation and protein content of the proapoptotic factor Bax. In addition, DPI signiﬁcantly induced the expression and phosphorylation of p53, which induces proapoptotic genes in response to DNA damage or irreparable cell cycle arrest. ROS have been implicated as a key factor in the activation of p53 by many chemotherapeutic drugs^[4].

in vivo

Diphenyleneiodonium (DPI), an NADPH oxidase inhibitor, inhibited the production of pro-inflammatory cytokines, (TNF-α and IL-6), reduced macrophage infiltration and classical polarization, and induced the ROS generation.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	ARPE-19 cells
	濃度	0.1, 1, and 10 μM
	反応時間	6, 12, 24, and 48 h
	実験の流れ	ARPE-19 cells are plated at 1×10⁶ cells per 60-mm dishes and incubated for 24 h. Cells are cultured in presence or absence of different concentrations of DPI in fresh DMEM/F12 medium supplemented with 10% FBS. After incubation, the cells are trypsinized, washed with phosphate-buffered saline (PBS) and the viable cells were scored by the trypan blue dye exclusion method using a hemocytometer.
動物実験	動物モデル	Male C57BL/6 mice
	投薬量	1 mg/kg
	投与方法	i.p.

参考

+ 展开

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner [ Cell Death Discov, 2024, 10(1):122]	PubMed: 38458999
Fusobacterium nucleatum-triggered neutrophil extracellular traps facilitate colorectal carcinoma progression [ J Exp Clin Cancer Res, 2023, 42(1):236]	PubMed: 37684625
Fusobacterium nucleatum-triggered neutrophil extracellular traps facilitate colorectal carcinoma progression [ J Exp Clin Cancer Res, 2023, 42(1):236]	PubMed: 37684625
Bi-fluorescent Staphylococcus aureus infection enables single-cell analysis of intracellular killing in vivo [ Front Immunol, 2023, 14:1089111]	PubMed: 36756129
Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH [ Apoptosis, 2023, 1-14.]	PubMed: 37306805
Multiparametric Profiling of Neutrophil Function via a High-Throughput Flow Cytometry-Based Assay [ Cells, 2023, 12(5)743]	PubMed: 36899878
Targeting NADPH Oxidase and Integrin α5β1 to Inhibit Neutrophil Extracellular Traps-Mediated Metastasis in Colorectal Cancer [ Int J Mol Sci, 2023, 10.3390/ijms242116001]	PubMed: 37958984
Imaging of innate immunity activation in vivo with a redox-tuned PET reporter [ Nat Biotechnol, 2022, 10.1038/s41587-021-01169-y]	PubMed: 35190688
Very-low-density lipoprotein receptor-enhanced lipid metabolism in pancreatic stellate cells promotes pancreatic fibrosis [ Immunity, 2022, 55(7):1185-1199.e8]	PubMed: 35738281
Elevated neutrophil extracellular traps by HBV-mediated S100A9-TLR4/RAGE-ROS cascade facilitate the growth and metastasis of hepatocellular carcinoma [ Cancer Commun (Lond), 2022, 10.1002/cac2.12388]	PubMed: 36346061

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。