Doxycycline

製品コードS5159 バッチS515903

印刷

化学情報

Synonyms

Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₂H₂₄N₂O₈

分子量

444.43

CAS No.

564-25-0

Solubility (25°C)*

体外

DMSO

89 mg/mL (200.25 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 Corn oil

3.25mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.
in vitro	100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used^[1]. Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes^[2].
in vivo	Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)^[2].

製品説明

Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.

in vitro

100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used^[1].

Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes^[2].

in vivo

Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines
	濃度	100 ng/mL, 1 µg/mL
	反応時間	24, 72, and 96 hours
	実験の流れ	Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.
動物実験	動物モデル	Male nu/nu mice
	投薬量	35 mg/pellet
	投与方法	s.c.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human [ Nat Genet, 2024, 56(1):124-135]	PubMed: 38195860
UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis [ Cell Rep, 2024, 43(2):113808]	PubMed: 38367236
The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epithelium [ Nat Commun, 2023, 14(1):6885]	PubMed: 37898598
IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma [ Nat Commun, 2023, 14(1):6781]	PubMed: 37880243
IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma [ Nat Commun, 2023, 10.1038/s41467-023-42545-3]	PubMed: 37880243
DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination [ Adv Sci (Weinh), 2023, 10(14):e2204438]	PubMed: 36965071
A kinome-wide CRISPR screen identifies CK1α as a target to overcome enzalutamide resistance of prostate cancer [ Cell Rep Med, 2023, 4(4):101015]	PubMed: 37075701
Targeting VCP potentiates immune checkpoint therapy for colorectal cancer [ Cell Rep, 2023, 42(11):113318]	PubMed: 37865914
NF-κB represses retinoic acid receptor-mediated GPRC5A transactivation in lung epithelial cells to promote neoplasia [ JCI Insight, 2023, 8(1)e153976]	PubMed: 36413416
APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress [ J Transl Med, 2023, 21(1):183]	PubMed: 36894994

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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