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Synonyms | Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C22H24N2O8 |
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分子量 | 444.43 | CAS No. | 564-25-0 | |
Solubility (25°C)* | 体外 | DMSO | 89 mg/mL (200.25 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor. |
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in vitro | 100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used[1]. Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes[2]. |
in vivo | Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)[2]. |
細胞アッセイ | 細胞株 | U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines |
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濃度 | 100 ng/mL, 1 µg/mL | |
反応時間 | 24, 72, and 96 hours | |
実験の流れ | Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter. |
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動物実験 | 動物モデル | Male nu/nu mice |
投薬量 | 35 mg/pellet | |
投与方法 | s.c. |
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Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human [ Nat Genet, 2024, 56(1):124-135] | PubMed: 38195860 |
UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis [ Cell Rep, 2024, 43(2):113808] | PubMed: 38367236 |
The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epithelium [ Nat Commun, 2023, 14(1):6885] | PubMed: 37898598 |
IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma [ Nat Commun, 2023, 14(1):6781] | PubMed: 37880243 |
IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma [ Nat Commun, 2023, 10.1038/s41467-023-42545-3] | PubMed: 37880243 |
DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination [ Adv Sci (Weinh), 2023, 10(14):e2204438] | PubMed: 36965071 |
A kinome-wide CRISPR screen identifies CK1α as a target to overcome enzalutamide resistance of prostate cancer [ Cell Rep Med, 2023, 4(4):101015] | PubMed: 37075701 |
Targeting VCP potentiates immune checkpoint therapy for colorectal cancer [ Cell Rep, 2023, 42(11):113318] | PubMed: 37865914 |
NF-κB represses retinoic acid receptor-mediated GPRC5A transactivation in lung epithelial cells to promote neoplasia [ JCI Insight, 2023, 8(1)e153976] | PubMed: 36413416 |
APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress [ J Transl Med, 2023, 21(1):183] | PubMed: 36894994 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。