Ethidium bromide

製品コードS3689 バッチS368901

化学情報

	Synonyms	Homidium bromide, EtBr, EB	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₁H₂₀N₃.Br
	分子量	394.31	CAS No.	1239-45-8
Solubility (25°C)*	体外	DMSO	78 mg/mL (197.81 mM)
		Water	7 mg/mL (17.75 mM)
		Ethanol	1 mg/mL (2.53 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
in vitro	Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis. It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized. The mode of binding of EtBr is intercalation between the base pairs. This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. The mobility of DNA was always less in the gels with EtBr^[1].

製品説明

Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

in vitro

Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis. It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized. The mode of binding of EtBr is intercalation between the base pairs. This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. The mobility of DNA was always less in the gels with EtBr^[1].

プロトコル(参考用のみ)

参考

[1] Sigmon J, et al. Electrophoresis. 1996, 17(10):1524-7.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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