Gamma-Oryzanol

製品コードS3957 バッチS395701

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C40H58O4
分子量 602.89 CAS No. 11042-64-1
Solubility (25°C)* 体外 DMSO 100 mg/mL (165.86 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Gamma-oryzanol (GO), a nutriactive phytochemical naturally occurring in crude rice bran oil, is an antioxidant compound used to treat hyperlipidemia, disorders of menopause and to increase the muscle mass.
in vitro From the proliferation assay using MTT reagent, the effect of gamma-oryzanol on DU145 and PC3 are slightly different with an IC50 after 7 days of exposure at 0.52±0.06 and 0.40±0.08 mg/ml, respectively. For in vitro condition in proliferation assay, IC50 values seem to be high in the scale of mg/ml. gamma-oryzanol may affect to prostate cancer cells through the down regulation of some antioxidant genes, CAT and GPX[1].
in vivo In tumor-bearing mice, gamma-oryzanol can reduce tumor mass associated with pro-angiogenic biomarkers[1]. Gamma-oryzanol is not carcinogenic and are safe for animals. In vivo trials proved gamma-oryzanol to be powerful antioxidant and also appears to be a very promising anticancerogenic compound. Supplementation of gamma-oryzanol in diabetic patients may improve metabolic dysfunctions such as hyperglycaemia, hypercholesterolaemia, hypertriglyceridaemia, insulin resistance and may alleviate diabetic complications resulting from lipid peroxidation and free radicals[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 human DU145 and PC3 prostate cancer cells
濃度 0.1-2.0mg/ml
反応時間 24 h
実験の流れ The prostate cancer cells at a density of 3.5×104 cells/well in serum-containing medium are seeded in 24-well microplates and grown to 80-90% confluency. Then, they are treated with gamma-oryzanol at various concentrations in a humidified atmosphere of 5%CO2 at 37°C for 24h. After the incubation, cells are washed twice and 150μl of culture medium containing 1mg/ml of MTT dye is added to each well and further incubated for 4h. The medium containing MTT dye is then replaced with 150μl of DMSO. The blue color of the oxidized MTT (formazan) as determined by measuring the absorbance at 570 nm using ELISA microplate reader.
動物実験 動物モデル C57BL/6J mice with ethanol-induced liver injury
投薬量 0.025 mmol/5 g ethanol per kg
投与方法 oral administration

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Rice Bran Extract Suppresses High-Fat Diet-Induced Hyperlipidemia and Hepatosteatosis through Targeting AMPK and STAT3 Signaling [ Nutrients, 2023, 15(16)3630] PubMed: 37630819
Rice Bran Extract Suppresses High-Fat Diet-Induced Hyperlipidemia and Hepatosteatosis through Targeting AMPK and STAT3 Signaling [ Nutrients, 2023, 15(16)3630] PubMed: 37630819
SChLAP1 promotes prostate cancer development through interacting with EZH2 to mediate promoter methylation modification of multiple miRNAs of chromosome 5 with a DNMT3a-feedback loop [ Cell Death Dis, 2021, 12(2):188] PubMed: 33589600

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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