|
受注：045-509-1970 |
技術サポート：[email protected] 平日9:00〜18:00 1営業日以内にご連絡を差し上げます |
化学情報
|
Synonyms | RS-21592, BW-759, 2'-Nor-2'-deoxyguanosine | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
| 化学式 | C9H13N5O4 |
|||
| 分子量 | 255.23 | CAS No. | 82410-32-0 | |
| Solubility (25°C)* | 体外 | DMSO | 51 mg/mL warmed with 50ºC water bath (199.81 mM) | |
| Water | Insoluble | |||
| Ethanol | Insoluble | |||
|
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
||||
溶剤液（一定の濃度）を調合する
生物活性
| 製品説明 | Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay. |
|---|---|
| in vitro | Ganciclovir is metabolized to the triphosphate form by primarily three cellular enzymes: (1) a deoxyguanosine kinase induced by CMV-infected cells; (2) guanylate kinase; and (3) phosphoglycerate kinase. [1] Ganciclovir is sufficient to induce cell death in most bystander cells cocultured with HSV-tk-expressing cells. [2] Ganciclovir significantly reduces DNA synthesis in the transformed cells, whereas Ganciclovir has little effect on DNA synthesis in the nontransformed cells. Ganciclovir exhibits a concentration-dependent reduction in the rate of elongation into mature DNA. [3] Ganciclovir induces cell cycle arrests rather than direct chemical effect on HSVtk-transduced B16F10 melanoma cells. [4] Ganciclovir produces only weak inhibition of DNA synthesis. Ganciclovir-treated cells accumulate in early S-phase and remained there until cell death, suggesting that ganciclovir incorporation in the DNA template is important for cytotoxicity. [5] Ganciclovir-induced apoptosis is due to incorporation of the drug into DNA resulting in replication-dependent formation of DNA double-strand breaks and, at later stages, S and G2/M arrest. Ganciclovir-provoked DNA instability is likely to be responsible for the observed initial decline in Bcl-2 level and caspase-9/-3 activation. [6] |
| in vivo | Antiviral drug ganciclovir (GCV) inhibits the proliferation of microglia in experimental autoimmune encephalomyelitis (EAE). GCV attenuates neuroinflammation and does not significantly restrain the peripheral immune response[7]. |
プロトコル(参考用のみ)
| 細胞アッセイ | 細胞株 | BV-2 cells |
|---|---|---|
| 濃度 | -- | |
| 反応時間 | 24 h | |
| 実験の流れ | To assess cell proliferation by thymidine incorporation, a total of 2 × 10<sup>4</sup> BV-2 cells were seeded in a 96-well plate for a maximum of 12 h in 10% FBS containing DMEM and then serum starved for an additional 12 h before the addition of varying concentrations of GCV for a total of 24 h. Cultures were pulsed for the final 8 h with 1 µCi/well [3H]thymidine before incorporated radioactivity was measured using a β plate scintillation counter. |
|
| 動物実験 | 動物モデル | C57BL/6 mice |
| 投薬量 | 100 mg/kg | |
| 投与方法 | i.p. |
参考
カスタマーフィードバック
-
Data from [Data independently produced by , , Sci Rep, 2017, 7: 43349]
Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：
| Cellular senescence in malignant cells promotes tumor progression in mouse and patient Glioblastoma [ Nat Commun, 2023, 14(1):441] | PubMed: 36707509 |
| Effects of cancer-associated fibroblasts deletion using HSVtk suicide system in OSCC [ Oral Dis, 2023, 10.1111/odi.14612] | PubMed: 37203377 |
| Tumor perivascular cell-derived extracellular vesicles promote angiogenesis via the Gas6/Axl pathway [ Cancer Lett, 2022, 524:131-143] | PubMed: 34678434 |
| Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2 [ JCI Insight, 2022, 7(14)e158207] | PubMed: 35708907 |
| Dioscin potentiates the antitumor effect of suicide gene therapy in melanoma by gap junction intercellular communication-mediated antigen cross-presentation [ Biomed Pharmacother, 2022, 150:112973] | PubMed: 35468581 |
| Safety, efficacy and delivery of multiple nucleoside analogs via drug encapsulated carbon (DECON) based sustained drug release platform [ Eur J Pharm Biopharm, 2022, S0939-6411(22)00047-9] | PubMed: 35314347 |
| Pericytes augment glioblastoma cell resistance to temozolomide through CCL5-CCR5 paracrine signaling [ Cell Res, 2021, 10.1038/s41422-021-00528-3] | PubMed: 34239070 |
| NUDT15-mediated hydrolysis limits the efficacy of anti-HCMV drug ganciclovir [ Cell Chem Biol, 2021, S2451-9456(21)00268-3] | PubMed: 34192523 |
| TGF-β/Alk5 signaling prevents osteoarthritis initiation via regulating the senescence of articular cartilage stem cells [ J Cell Physiol, 2021, 10.1002/jcp.30231] | PubMed: 33452687 |
| 6-Thioguanine Inhibits Herpes Simplex Virus 1 Infection of Eyes [ Microbiol Spectr, 2021, 9(3):e0064621] | PubMed: 34730435 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。