Higenamine hydrochloride

製品コードS3960 バッチS396001

印刷

化学情報

Chemical Structure Synonyms Norcoclaurine HCl, (+-)-Demethylcoclaurine hydrochloride Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H17NO3.HCl
分子量 307.77 CAS No. 11041-94-4
Solubility (25°C)* 体外 DMSO 61 mg/mL (198.19 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Higenamine (Norcoclaurine, (+-)-Demethylcoclaurine), also known as Norcoclaurine HCl, is a non-selective β2 adrenoceptor agonist which is a chemical compound naturally occurring in a number of plants.
in vitro Higenamine is a potential β2-adrenoceptor agonist. As a potent cardiotonic and vasodilator, It is also an α1 antagonist and a weak α2 agonist[1]. Higenamine decreases the intracellular dopamine content dose-dependently and shows 55.2% inhibition of dopamine content in PC12 cells at a concentration of 2OM with 24h incubation. The IC50 value of higenamine for inhibiting dopamine biosynthesis in PC12 cells is 18.2 μM. Dopamine content is lowered and reaches minimal level at 12—24h after exposure to higenamine[2].
in vivo Higenamine can produce relaxation in tracheal muscle[1]. Higenamine has anti-thrombotic effects in both mouse acute thrombosis model and rat arterio-venous shunt (AV-shunt) models. The oral administration of higenamine (50 or 100 mg/kg) increases the recovery rates from the acute thrombotic challenge in mice and lowers the weight of thrombus formed inside the AV-shunt tube in rats[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 PC12 cells
濃度 5-20 μM
反応時間 12-48 h
実験の流れ

PC12 cells are maintained routinely in RPMI 1640 medium supplemented with 10% heat-inactivated horse serum and 5 % fetal calf serum pius 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C. The PC12 cells (ca. 1×105 cells/cm2) are treated with higenamine (5-2O μM) and then incubated for 12 (-48) h. The cells (ca. 1.5-2 × 105 cells/cm2) are harvested with phosphate buffered saline (PBS) and the pellet extract is used for the measurement of dopamine content, and TH and AADC activities.
動物実験 動物モデル mice
投薬量 50 or 100 mg/kg
投与方法 oral

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Higenamine mitigates interleukin-1β-induced human nucleus pulposus cell apoptosis by ROS-mediated PI3K/Akt signaling [ Mol Cell Biochem, 2021, 10.1007/s11010-021-04197-z] PubMed: 34146182
Chronic stress promotes glioma cell proliferation via the PI3K/Akt signaling pathway [ Oncol Rep, 2021, 46(3)202] PubMed: 34296295

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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