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Synonyms | Hygrovetine | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C20H37N3O13 |
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分子量 | 527.52 | CAS No. | 31282-04-9 | |
Solubility (25°C)* | 体外 | Water | 100 mg/mL (189.56 mM) | |
DMSO | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Hygromycin B (Hygrovetine), a selective antibiotic that is effective on most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome. |
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in vitro | Hygromycin B has a single binding site within the 30S, it locates in the major groove of the helix very close to the helical axis, and makes contact with nucleotides from both RNA strands in the region 1490-1500 and 1400–1410. Ring I of HygB is involved in nonsequence-specific interactions with the backbone phosphate oxygen atoms of G1494 in addition to base-specific interactions with G1494 and U1495. Rings II and III make weak base-specific hydrogen bonds to both C1404 and U1498, but their main role apparently is to position ring IV for interaction with bases in the 1496–1498 region. Ring IV of HygB comes within 4A of the second base of the P site bound mRNA codon. [1] Hygromycin B (0.38 mM) completely halts yeast cell growth in rich media. Hygromycin B strongly block Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast. Hygromycin B inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. [2] Hygromycin B inhibits (80%) the elogation-factor-(EF)G-plus-GTP-dependent reaction of either AcPhe-tRNA or natural peptideyl-tRNA with puromycin in Escherichia coli cell-free systems. Hygromycin B blocks the nascnet peptide chains of either purified endogenous E. coli polysomes or poly(uridylic acid)-programmed ribosomes during polypeptide synthesis. Hygromycin B inhibits the non-enzymic translocation and the release of AcPhe-tRNA from the ribosomal acceptor site promoted by depletion of NH4 ions. [3] Hygromycin B is used for resistance selection in mouse L cells transfected with Escherichia coli genes coding for resistance to the aminocyclitol antibiotics hygromycin B. [4] Hygromycin B is used as a selectable marker for T-DNA transfer and integration in the ectomycorrhizal fungus Suillus bovinus. [5] |
細胞アッセイ | 細胞株 | Yeast cells |
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濃度 | 0.38 mM | |
反応時間 | ||
実験の流れ |
APOBEC3B coordinates R-loop to promote replication stress and sensitize cancer cells to ATR/Chk1 inhibitors [ Cell Death Dis, 2023, 14(6):348] | PubMed: 37270643 |
Detection of Alternative End-Joining in HNSC Cell Lines Using DNA Double-Strand Break Reporter Assays [ Bio Protoc, 2022, 12(17)e4506] | PubMed: 36213110 |
CGAS is a micronucleophagy receptor for the clearance of micronuclei [ Autophagy, 2021, 1-17] | PubMed: 33752561 |
The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NFκB transcripts to dampen antiviral innate immunity [ PLoS Pathog, 2021, 17(4):e1009530] | PubMed: 33909701 |
[ Cell Res, 2020, ] | PubMed: 32839552 |
SETDB1-Mediated Cell Fate Transition between 2C-Like and Pluripotent States. [ Cell Rep, 2020, 30(1):25-36] | PubMed: 31914391 |
[ Sci Rep, 2020, ] | PubMed: 32848164 |
Identification of MRP4/ABCC4 as a Target for Reducing the Proliferation of Pancreatic Ductal Adenocarcinoma Cells by Modulating the cAMP Efflux. [ Mol Pharmacol, 2019, 96(1):13-25] | PubMed: 31043460 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。