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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C12H22ClN3OS |
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分子量 | 291.84 | CAS No. | 1803605-68-6 | |
Solubility (25°C)* | 体外 | DMSO | 58 mg/mL (198.73 mM) | |
Water | 58 mg/mL (198.73 mM) | |||
Ethanol | 58 mg/mL (198.73 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | ICCB-19 hydrochloride is an inhibitor of TNFRSF1A Associated Via Death Domain (TRADD) with IC50 of 1.12 μM and 2.01 μM for protecting Velcade-induced apoptosis in Jurkat cells and protecting RDA in MEFs, respectively. ICCB-19 indirectly inhibits Receptor-interacting serine/threonine-protein kinase 1 (RIPK1). ICCB-19 effectively induces autophagy. |
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in vitro | ICCB-19 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N), which interacts with the C-terminal domain (TRADD-C) and TRAF2 to modulate the ubiquitination of RIPK1 and beclin 1.[2] |
細胞アッセイ | 細胞株 | HEK293T cells, MEFs, Jurkat cells, SH-SY5Y cells, HT-29 cells, U-937 cells, H4 cells and HeLa cells |
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濃度 | 10 μM | |
反応時間 | -- | |
実験の流れ | The rates of cell death were measured in triplicate or quadruplicate in a 96-well or 384-well plate using SYTOX Green Nucleic Acid Stain or ToxiLight Non-destructive Cytotoxicity BioAssay Kit. The intensity of luminescence was determined in an EnSpire Multimode Plate Reader. Cytotoxicity was expressed as percentages of cell death per well after deducting the background signal in non-induced cells and compared to that of the maximal cell death with 100% Lysis Reagent. |
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Inhibition of TRADD ameliorates chondrocyte necroptosis and osteoarthritis by blocking RIPK1-TAK1 pathway and restoring autophagy [ Cell Death Discov, 2023, 9(1):109] | PubMed: 37002200 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。