ID-8

製品コードS7327 バッチS732701

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₆H₁₄N₂O₄
	分子量	298.29	CAS No.	147591-46-6
Solubility (25°C)*	体外	DMSO	60 mg/mL (201.14 mM)
		Ethanol	2 mg/mL (6.7 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.
in vitro	ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. ^[1] ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs. ^[2]

製品説明

ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.

in vitro

ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. ^[1] ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs. ^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human embryonic stem cells
	濃度	~10 μM
	反応時間	7 days
	実験の流れ	Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

In vivo drug discovery for increasing incretin-expressing cells identifies DYRK inhibitors that reinforce the enteroendocrine system [ Cell Chem Biol, 2022, 29(9):1368-1380.e5]	PubMed: 35998625

In vivo drug discovery for increasing incretin-expressing cells identifies DYRK inhibitors that reinforce the enteroendocrine system [ Cell Chem Biol, 2022, 29(9):1368-1380.e5]

PubMed: 35998625

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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