iHAP1

製品コードS9695 バッチS969501

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C20H14ClNO2S
分子量 367.85 CAS No. 105925-39-1
Solubility (25°C)* 体外 DMSO 20 mg/mL (54.36 mM)
Ethanol 5 mg/mL (13.59 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 iHAP1 is a well-established microtubule poison, blocks microtubule polymerization fully at 5 µM which is independent of deregulated transcription, causes marked sensitization when deleting Rod1 gene in HeLa cells.
in vitro

The depletion of Rod1 and TACC3 results in sensitization to iHAP1, which is caused by chemogenic interaction between Rod1 and iHAP1. As for the RPE1‐hTERT P53−/− background, deletion of Rod1 in HeLa cells causes marked sensitization to iHAP1.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 RPE1‐hTERT P53−/− Flag‐Cas9 cell line
濃度 0.16-1 µM
反応時間 12 days
実験の流れ

The Sulforhodamine B (SRB) proliferation assay was used to assess in vitro cytotoxicity of the compounds. Cells were treated with sublethal drug concentrations (LD20) for 12 days from the day of seeding. If necessary, transient siRNA transfection was performed 3 days before drug treatment. After 12 days, medium was removed from the wells and cells were washed once with PBS and fixed in prechilled 10% trichloroacetic acid (TCA) for 30 min at 4°C. Cells were subsequently washed two times with double‐distilled water and stained with 0.4% SRB (1% acetic acid for 20 min at room temperature protected from light). SRB was then removed, and cells were washed four times in 1% acetic acid. After complete light‐protected drying, SRB was dissolved by addition of 10 mM Tris pH 8 and gentle shaking at room temperature for 2 h. The absorbance at A510 was read. Percentage of cell growth inhibition was calculated. The IncuCyte® live‐cell analysis system was additionally used to real‐time track the growth of the cells subjected to the different treatments.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Non-catalytic allostery in α-TAT1 by a phospho-switch drives dynamic microtubule acetylation [ J Cell Biol, 2022, 221-11e202202100] PubMed: 36222836

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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