IKE(Imidazole ketone erastin)

製品コードS8877 バッチS887704

印刷

化学情報

Chemical Structure Synonyms PUN30119 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C35H35ClN6O5
分子量 655.14 CAS No. 1801530-11-9
Solubility (25°C)* 体外 DMSO 100 mg/mL (152.63 mM)
Ethanol 100 mg/mL (152.63 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 IKE(Imidazole ketone erastin) is a potent, selective, and metabolically stable system xc– inhibitor and inducer of ferroptosis.
in vitro

Imidazole ketone erastin (IKE) is an erastin analog with nanomolar potency, high metabolic stability, and intermediate water solubility. Its treatment potently reduces DLBCL cell number[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 DLBCL cell line
濃度 0-100 μM
反応時間 24 h
実験の流れ

DLBCL cells are plated at 10,000 cells per well in white 384-well plates (32 μL per well) in technical duplicates and incubated overnight. The cells are then treated with 8 μL medium containing a two-fold dilution series of vehicle (DMSO), IKE (starting from 100 μM) with or without Fer-1 (starting from 200 μM). After 24 h incubation, 40 μL of 50% CellTiter-Glo / 50% cell culture medium is added to each well and incubated at room temperature with shaking for 15 min. Luminescence is measured. All cell viability data are normalized to the DMSO vehicle condition. Experiments are performed three independent times with different passages for each cell line. From these data, dose-response curves and IC50 values are computed.
動物実験 動物モデル Non-obese diabetic/ severe combined immunodeficiency (NOD/SCID) mice
投薬量 50 mg/kg
投与方法 IP, IV and oral administration

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137] PubMed: 38642502
Augmented ERO1α upon mTORC1 activation induces ferroptosis resistance and tumor progression via upregulation of SLC7A11 [ J Exp Clin Cancer Res, 2024, 43(1):112] PubMed: 38610018
CYLD regulates cell ferroptosis through Hippo/YAP signaling in prostate cancer progression [ Cell Death Dis, 2024, 15(1):79] PubMed: 38246916
Mutational signature-based identification of DNA repair deficient gastroesophageal adenocarcinomas for therapeutic targeting [ NPJ Precis Oncol, 2024, 8(1):87] PubMed: 38589664
Phospholipase PLA2G7 is complementary to GPX4 in mitigating punicic-acid-induced ferroptosis in prostate cancer cells [ iScience, 2024, 27(5):109774] PubMed: 38711443
Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade [ Nat Commun, 2023, 14(1):4758] PubMed: 37553341
Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade [ Nat Commun, 2023, 14(1):4758] PubMed: 37553341
RB1-deficient prostate tumor growth and metastasis are vulnerable to ferroptosis induction via the E2F/ACSL4 axis [ J Clin Invest, 2023, 133(10)e166647] PubMed: 36928314
RB1-deficient prostate tumor growth and metastasis are vulnerable to ferroptosis induction via the E2F/ACSL4 axis [ J Clin Invest, 2023, 133(10)e166647] PubMed: 36928314
Targeting N-glycosylation of 4F2hc mediated by glycosyltransferase B3GNT3 sensitizes ferroptosis of pancreatic ductal adenocarcinoma [ Cell Death Differ, 2023, 30(8):1988-2004] PubMed: 37479744

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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