Isatin

製品コードS4717 バッチS471701

印刷

化学情報

Chemical Structure Synonyms 2,3-Indolinedione Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C8H5NO2
分子量 147.13 CAS No. 91-56-5
Solubility (25°C)* 体外 DMSO 29 mg/mL (197.1 mM)
Ethanol 10 mg/mL (67.96 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Isatin (2,3-Indolinedione) is an endogenous MAO inhibitor and more active against MAO-B than MAO-A
in vitro Isatin induces apoptosis of MCF-7 cells. Bcl-2 expression is decreased and the ratio of Bcl-2 to Bax is significantly decreased by isatin. The mitochondrial transmembrane potential is markedly decreased and the release of cytochrome c into the cytosol is elevated following treatment with isatin. At the same time, caspase-9 and -3 are stimulated, followed by the degradation of ICAD, a caspase-3 substrate. Isatin and its analogs inhibits the proliferation of some cancer cells, including colon HT29, breast MCF-7, lung A549 and melanoma UACC903 cells and is a dual inhibitor of tubulin polymerization and the Akt pathway[4].
in vivo Isatin is an endogenous indole that is increased in stress, inhibits monoamine oxidase (MAO) B and improves bradykinesia and striatal dopamine levels in rat models of Parkinson's disease. Isatin has a distinct and discontinuous distribution in rat brain and other tissues; the highest concentrations in the brain are found in the hippocampus and cerebellum. In rodent models isatin has been shown to cause a widespectrum of dose-dependent physiological and biological actions, such as anxiogenic and sedative effects, memory dysfunction and inhibition of food and water intake. Significantly, isatin readily crosses the blood-brain barrier so that a peritoneal dose of 100 mg/kg would result in a concentration of about 120 μM in the rat brain. This concentration would increase further with repeated injections[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 human neuroblastoma (SH-SY5Y) cells
濃度 0.1-400 μM
反応時間 24 or 48 h
実験の流れ Cell viability was estimated by a colorimetric method, which is based on the ability of cellular dehydrogenases of viable cells to reduce MTT from a yellow watersoluble dye to a dark blue insoluble formazan product. SHSY5Y cells were seeded in 96-well plates at 4×104 cells/well/(100 ml) and allowed to attach. The cells were then treated with isatin and returned to the incubator for 24 or 48 h. MTT 25 μl (5 mg/ml) was added to all wells and allowed to incubate in the dark at 37℃ for 2 h followed by cell lysis. The plates were read with an OPTImax microplate reader at wavelength of 562 nm. Controls included untreated cells and medium alone, with all MTT assays performed in triplicate.
動物実験 動物モデル Albino rats of the Fisher strain
投薬量 100 mg/kg
投与方法 i.p

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Genome-wide CRISPR-Cas9 screening identifies the CYTH2 host gene as a potential therapeutic target of influenza viral infection [ Cell Rep, 2022, 38(13):110559] PubMed: 35354039

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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