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Synonyms | MIF Antagonist | Storage (From the date of receipt) |
3 years -20°C powder | ||||||||
化学式 | C12H13NO4 |
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分子量 | 235.24 | CAS No. | 478336-92-4 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 47 mg/mL (199.79 mM) | ||||||||
Ethanol | 47 mg/mL (199.79 mM) | ||||||||||
Water | Insoluble | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM. |
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in vitro | ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages[2]. |
in vivo | ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP)[2]. It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. ISO-1 attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema[3]. ISO-1 protects photoreceptors and reduces gliosis in experimental retinal detachment[4]. |
細胞アッセイ | 細胞株 | RAW 267.4 macrophages |
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濃度 | 1-100 μM | |
反応時間 | 30 min | |
実験の流れ | RAW 267.4 macrophages were treated with various concentrations of ISO-1 (1-100 μM) 30 min prior to LPS addition. Macrophages were collected 2 h after activation with LPS and washed one time in PBS (pH 7.4). Nuclear extract was isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents. |
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動物実験 | 動物モデル | Male Balb/C mice, ∼8 weeks old, subjectd to endotoxemia or cecal ligation and puncture (CLP) |
投薬量 | 3.5-35 mg/kg | |
投与方法 | i.p. |
MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinoma [ iScience, 2023, 26(8):107273] | PubMed: 37520719 |
MIF Increases sFLT1 Expression in Early Uncomplicated Pregnancy and Preeclampsia [ Int J Mol Sci, 2023, 24(12)10050] | PubMed: 37373198 |
Therapeutic targeting of tumor spheroids in a 3D microphysiological renal cell carcinoma-on-a-chip system [ Neoplasia, 2023, 10.1016/j.neo.2023.100948] | PubMed: 37944353 |
Ischemic stroke protected by ISO-1 inhibition of apoptosis via mitochondrial pathway [ Sci Rep, 2023, 13(1):2788] | PubMed: 36797398 |
On the origin of metastases: Induction of pro-metastatic states after impending cell death via ER stress, reprogramming, and a cytokine storm [ Cell Rep, 2022, 38(10):110490] | PubMed: 35263600 |
Exosomes from adipose-derived stem cells regulate M1/M2 macrophage phenotypic polarization to promote bone healing via miR-451a/MIF [ Stem Cell Res Ther, 2022, 13(1):149] | PubMed: 35395782 |
Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI [ Cell Death Discov, 2022, 8(1):61] | PubMed: 35165294 |
MIF/CXCR4 signaling axis contributes to survival, invasion, and drug resistance of metastatic neuroblastoma cells in the bone marrow microenvironment [ BMC Cancer, 2022, 22(1):669] | PubMed: 35715791 |
Isopsoralen ameliorates rheumatoid arthritis by targeting MIF [ Arthritis Res Ther, 2021, 23(1):243] | PubMed: 34535196 |
Utx Regulates the NF-κB Signaling Pathway of Natural Stem Cells to Modulate Macrophage Migration during Spinal Cord Injury [ J Neurotrauma, 2021, 38(3):353-364] | PubMed: 32977735 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。